264 
J. 0. Wakelin Barratt and G. Arnold 
2. Granules and globales of fat are not seen in spermatogonia, in 
spennatocytes or in cells of Sertoli, bat tliey are always seen in yonng 
spermatids, and are abundant in the cytoplasm of spermatids in which 
the spermatozoal head has made its appearance, and also in the cast-off 
cytoplasm of spermatids. Their appearance in moderate degree is normal; 
after the application of X rays their numbers undergo considerable in- 
crease. The size of the fat globules varies from 2 u or less to 4» or more. 
Tliis form of degeneration was usually met with between the 4th and 
9th days. The large fatty globules, presumably representing degenerated 
spermatids, normally found near the basement membrane, already 
referred to on p. 256, are also markedly increased in number after the 
application of X rays, the increase appearing after the 3rd day. Fatty 
globules of small size are also met with in small numbers in multi- 
vacuolate cells. 
3. Vacuolation of the cytoplasm, which is rarely met with, occurs 
in the cells of Sertoli. It has beeil observed on the 3rd to the 7th day 
and is characterized by the appearance of well defined, more or less 
globular, apparently fluid collections 3 [i to 5 // in diameter. 
Nucleus. The non-dividing nucleus: 1. may become multiple; 
2. may be the seat of, or become involved in, vacuole formation resulting 
in compression of the nucleoplasm; 3. may undergo necrosis at the same 
time as the cytoplasm. 
1. Multiplication of the nuclei is the result of irregulär mitotic division 
of spermatocytes of the first order and of amitosis of spermatocytes of 
the second order. Its makes its appearance after the 3rd day and disap- 
pears after the 7th day. The nuclei are irregulär in size, but remain 
more or less globular in aspect. In number they ränge from two to four, 
occasionally five to eight (p. 261). 
2. The formation of vacuoles in connection with the nucleus is re- 
ferred to on p. 266. Compression of the nucleus, which occurs as the 
result of vacuole formation, is generally eccentric in character. Beyond 
this and darker staining, due to the approximation of the chromatin 
masses, no further cliange occurs. Compression of nuclei is seen in sper- 
matocytes of the first order and spermatids ; in the former on the 3rd day 
to the 9th day, in the latter between the 4th and 9th days. Alteration 
in the chromatin of the nucleus occur in connection with the change next 
to be described. The nuclear membrane disappears or becomes indistinct ; 
the chromatin network is not well defined. 
3. Necrosis, characterized by diffuse cloudiness of the nucleus, the 
hyaloplasm of which stains by basic instead of acid dyes, has been ob- 
