Cell Changes in the Testis due to X Rays. 
275 
Fig. 22. Cell lying 15 u from the basement membrane. The characters of the 
ceU are so altered that it is not possible to identify it with certainty, but it resembles a 
spermatocyte of the second Order more than any other cell form. The nucleus con- 
tains a large vacuole completely surrounded by the remains of the nucleoplasm. Below 
is a black granule, contained in a small vacuole, completely embedded in the Condensed 
nuclear remains; the origin of this structure is uncertain. Free in the cytoplasm are 
two small granules derived from the chromatoid body. The cytoplasm, which is granulär 
in appearance, does not present any archoplasm. 
Fig. 23. Cell situated at the lumen of a seminiferous tubule. ? Altered spermato- 
cyte of the second order. A large archoplasmic vesicle is formed, to the left of which 
are seen the cup shaped remains of the nucleus still exhibiting a network arrangement 
of the chromatin. Below and to the left, situated in the cytoplasm, which is coarsely 
granulär, two darkly staining masses representing chromatoid bodies are seen. Lying 
upon the cup shaped nuclear remains is a small vesicle containing a central granule. 
Xo archoplasm is recognizable. 
Fig. 24. Large cell mass presenting many nuclei each containing a large vacuole ; 
it is situated about 25 ju from the basement membrane of a seminiferous tubule. The 
number of nuclei is altogether 9, but only 5 are represented. The nuclear chromatin, 
darkly stained, is spread out over the greater part, but not the whole of the surface 
of the vacuoles. In addition, there are about 12 smaller masses, of which only 9 are 
shown in the sketch. Some of these appear to be chromatoid bodies ; others may also 
be of the same nature. The cytoplasm, which is dark, is finely granulär. 
Fig. 25. Large multivacuolated cell mass, situated 15 u from the basement 
membrane of a seminiferous tubule. Five vacuoles of varying size are seen; the 
larger of these have obviously each arisen within a nucleus, the chromatin of which 
has been compressed to form a thin covering for the vacuole; the vacuoles are filled 
with clear structureless material presumably fluid. The cytoplasm, which is finely 
granulär and is degenerated, taking up basic dyes more readily than does healthy cyto- 
plasm, contains numerous darkly staining granules, presenting little evidence of structure. 
The granules shown in the sketch in connection with the largest vacuole lie outside 
the structureless material with which it is filled. 
Fig. 26. Spermatid situated about 15 u from the basement membrane. In the 
nucleus of this cell a large vacuole situated excentrically is completely embedded, the 
nuclear chromatin being considerably compressed. To the left is an archoplasmic mass, 
between which and the nucleus is an archoplasmic vesicle containing an intermediate 
body. Above, a chromatoid body is seen breaking up into small darkly stained frag- 
ments. The cytoplasm presents a granulär aspect and is shreddy at the edges. 
Fig. 27. Young spermatid, situated close to the basement membrane. An 
enlarged archoplasmic vesicle has pressed upon the nucleus, which has become cup 
shaped. Xo intermediate body is recognizable. Above and to the right an archoplasm 
is seen. Below and to the left several small granules are visible, probably representing 
a chromatoid body. The cytoplasm, which is granulär, presents a sharp outline. 
Fig. 28. Cell situated about 12 [x from the basement membrane. This cell is a 
spermatid with an enlarged archoplasmic vesicle compressing the nucleus, the chromatin 
of which is disposed as a darkly stained cup shaped mass lying below and to the left. 
Near this a small rounded mass, representing a chromatoid body, is seen surrounded 
by a clearer area. Close by is a smaller darkly stained granule. The cytoplasm is 
finely granulär. An archoplasm can be seen above and to the left. 
