236 
SIDNEY I. KORNHAUSER 
this variation, which had already occupied the attention of such 
pioneers as Carnoy, La Valette, St. George and Sinety, should 
be produced by parasitism. 
Brauns (G2), in his study of the oogenesis of Forficula auric- 
ularia, believes that the haploid number in the female is 13. 
He quotes Professor Ludwig Will of Rostock as saying that the 
male diploid number of Forficula is 25, and the female diploid 
number 26; but that some males show 24 chromosomes in their 
spermatogonia. If is a striking coincidence that my counts on 
Anisolabis are in very close agreement with the results of Pro- 
fessor Will. 
Randolph (^08) worked on Anisolabis and described 24 chromo- 
somes as the diploid number in both sexes. In the primary 
spermatocytes she pictures a pair of almost equal hetero chromo- 
somes which lag in the anaphase of the first maturation mitosis, 
but her results are not at all in accord with those described in 
the present paper. 
3. METHODS 
The material studied consisted of gonads and embryos. 
Nymphs were found best for the study of the germ cells. The 
animals were all collected at or near Cold Spring Harbor, Long 
Island, during June, July and August of several summers since 
1915. 
The best fixatives were Flemming’s fluid (strong), Bouin’s 
fluid and Benda’s modification of Flemming’s fluid. Various 
stains were employed. The Flemming material was stained in 
Heidenhain’s haematoxylin, counterstained with Orange G or 
with safranin and lichtgrlin. The Benda material was stained 
either with alizarin and crystal viplet, or with methyl green and 
acid fuchsin of Bensley. These last two were especially valuable 
in the study of the xxy-complex. 
Smears of testes were made in a moist chamber, immediately 
exposed to osmic fumes for a few seconds, and then immersed 
in a fixing fluid; care being taken to avoid drying. Such 
smears were then treated and stained like sections. 
