242 
SIDNEY I. KORNHAUSER 
strepsinema. Finally in the later strepsistene stage, when the 
chromatic spherule is given off, the y-element stains as deeply 
as the two x-elements. In crystal violet + alizarin the y-element 
is purple, and the xx-element brown with small purple granules 
in it; whereas the nucleolar sphere of the late strepsinema is 
deep purple. In methyl green + acid fuchsin, the y-element is 
red, and the xx-element green. As the two fuse, in stages 
corresponding to figures 25 and 26, the y-element gradually loses 
its red color and becomes green, while the extruded nucleolar 
spherule is a deep red. We must, I believe, assume that the 
extrusion of this nucleolar spherule is in a vital way connected 
with the change in ^^stainability’’ of the y-element. 
The twelve chromosomes of the primary spermatocyte meta- 
phase (Plate XXV, figs. 28, 29) are so widely separated that, in 
lateral views with very high magnification, one may focus sharply 
on each element in the spindle. Good lateral views of the xxy- 
hexad may be obtained not only in metaphase plates (figs. 30-33) 
but also in anaphase stages (Plate XXVI, figs. 37, 38). Figure 
34 (Plate XXV) shows the twelve elements of the metaphase plate 
viewed laterally, the chromosomes of the several foci being here 
transposed into a single row from the camera lucida drawing. 
The hexad is on the extreme right. Figure 35 represents a 
corresponding stage, but taken from a smear slide. The contents 
of the cell were so spread out that a single focus di^layed 
all the chromosomes with no overlapping. In the smear slides 
the chromatic elements appear smaller but retain all the features 
seen in sections, and offer a very good check upon the latter. 
The attachment of the y-element and the xx-element seems 
y 
XX 
Various arrange- 
to be either terminal x or lateral 
l X J 
ments of the elements taken from metaphases and anaphases are 
seen in figures 30-38. Figure 36 (Plate XXV) shows seven 
separate xxy-hexads, the y-element in all cases being uppermost 
in the figures. 
In the first meiotic division (Plate XXVI, figs. 37-41) the 
y-chromosome passes to one pole and the double x-chromosome 
