270 
Sidnev I. Kornhauser 
Figure 137 (Plate XX) represents an early auapliase of the first 
spermatocyte division. On the left is seeii the macrochromosonie geminus 
with two fibers stiU connecting its component halves. In the middle is 
the xy pair, the connecting Strand having just broken. Figure 138 
(Plate XX) shows a cell located at the edge of a section stained in 
crystal-violet after flsation in Hermann’s fluid. The xy pair has stained 
deep purple, the autosomes niuch lighter. The xy connecting strand also 
shows well. The Separation of all the chromosomes is synchronous 
(Plate XX, Fig. 139), the xy pair neither preceding nor lagging behind the 
autosomes. As the chromosomes near the poles of the spindle (Plate XX, 
Fig. 140), they appear much shorter in side view and often form 
a nearly flat plate; this enables one to study them in polar views with- 
out confusion. Figure 141 (Plate XX) represents a late anaphase 
group seen in end view. It is composed of ten chromosomes, the macro- 
chromosome, longitudinally split, eight smaller chromosomes also split, 
and one showing no division. IVhether the undivided chromosome is the 
allosome, which is slow in dividing, cannot be stated with certainty, but 
in no case was it possible to count niore than nine chromosomes which 
showed the future line of Separation of the second division. There is 
no interkinetic period, the second spermatocyte division foUowing imme- 
diately upon the first. Often metaphase stages of the second division 
and anaphases of the first division are found in the same cyst. 
B. Enchenopa eurvata. 
As in binotata, there are ten chi'omosonies in the metaphase of the fhst 
spermatocyte division (Plate XXI, Figs. 142, 143). Side views (Plate XXI, 
Figs. 144—147, consecutive sections of one cell) show the macro- 
clironiosome paii', eight smaller gemini and one smgle spherical chromo- 
some, the a>-element, which is usuaUy peripheral in position (Plate XXI, 
Figs. 142, 147 x) and nearer one pole than the other (Figs. 148, 149 x). 
This ic-element may also be distinguished by the thick linin fibers which 
connect it to the neighboring gemini. These fibers stam niore deeply 
than those connecting the autosomes with one another and play an im- 
portant part in the behavior of the a;-chromosome during the first divi- 
sion. They are, I believe, the same fibers that were seen in the prophase 
of the first spermatocyte (Plate XX, Fig. 101, 120) and are referable to 
the Connection which existed between the ic-chromosome and the chro- 
matic nucleoli which, after the bouquet stage, became connected with 
the autosome loops (Plate XX, Figs. 85, 92, 93). 
