400 
H. E. Jordan 
The most important cpiestion here concerns the true relation of the iiu- 
cleoli to the chroniosomes. Careful Observation reveals three Cardinal 
facts; 1) origin of smaller froin larger chromatic nucleoli; 2) dose spatial 
relationship of chromosomes to nucleoli, frequently direct contact; 3) 
correspondence in size between smaUest nucleoli (products of nucleolar 
budding) and the constituent granules (chromomeres?) of the chromo- 
Fig. 7. 
somes. 
It must be emphasized, however, that not aU of the nucleolar sub- 
stance can be thought of as passing into the chromosomes. Here, as in 
the prematuration stages of many eggs (e. g., Asterias foriesii), there is 
suggested a condition of binuclearity in the sense that a portion of the 
chromatic constituents of the nucleus passes into chroniosomes and clearly 
indicates its reproductive nature, while a portion 
is resorbed by cytoplasm (vegetative nature?). An 
alternative interpretation might regard all chroma- 
tin as nutritive; for the facts above outlined do 
not unequivocally indicate whether the nucleolar 
chromatin simply contributes material to the chro- 
mosomes or whether it actually constructs them. 
That the nucleolus is simply a store-house of 
material for supplying nutriment to the chromatin 
has been urged hy Flejiming^), Bambeke^), Kor- 
scHELT^), and more recently by Lubosch^). 
Where the nucleolus itself is chromatic and che- 
mically (according to staining reaction) indi- 
stinguishable from the chromatin of the chromosomes, and where the 
former contributes to the formation of the latter as in Asterias, CribreTla, 
Echinaster, and many other forms, the chromatin may perhaps in part 
at least be thought of as nutritive. 
In a recent paper Sheppard^) has reported some very interesting 
Ulustration from another egg 
to Show how the chromosomes 
appear to arise hy a process 
of alignment of nucleolar bnds. 
X 1500. 
1) Flemmixg, W. Zellsubstanz, Kern und Zellteilung. Leipzig, C. F. W. Vogel. 
1882. 
2) Bambeke, C. Vax. Etat actuel de nos connaissances sur la structure du noyau 
cellulaire ä l’etat de repos. Ann. Soc. de Med., Gand. 1885. 
3) Korschelt, E. Über Kernteilung, Eireifung und Befruchtung bei Ophryo- 
trocha puerilis. Zeitschr. f. wiss. ZooL, Bd. 54. 1895. 
Lubosch, W., Über die Eireifung der Metozoen usw. Merkel u. Bonnet, Er- 
gebnisse der Anat. u. Ent\nckl. Vol. 11. 1902. 
5) Sheppard, E. J. The Disappearance of the Nucleolus in Mitosis. Joum. 
Royal Micr. Soc Oktober 1909 p. 551. 
