The relation of nucleoli to chromosomes in the egg of Cribrella sanguineolenta etc. 401 
facts bearing on this matter in the dividing cells of the growing roots of 
Hyacinthus and Allium and in those of epidermis of the tadpole. The 
delicate observations were possible only as the result of a faultless technic. 
The essential facts are these : a) The nucleoli of quiescent cells stain densely 
(in iron-hematoxylin or thionin) and stand out clearly from the less con- 
Fig. 8. Fig. 9. 
Illustration from a third egg to show extremely long chromosomes. In every instance they assume an 
intimate spatial relationship with the nucleoli. In Äuerbach's stain the chromosomes show a compound 
structure of a red-staining gronnd substance (linin?) onto which are placed the green spheric granulös 
(chromomeres?). X 1500. Fig. 9. Illustration of a region of another egg to show especially the smaller 
nucleolar buds. The chromosomes are similar to those above described, and show the same intimate 
relationship to the nucleoli. X 1500. 
spicuous chromatic granules and linin network. b) At the beginning of 
the spireme stage the nucleoli take the stain less deeply in the central 
portion. c) Düring later spireme stages, the staining power of the entire 
nucleolus diminishes and signs of fragmentation appear at the periphery. 
d) There is an intimate association of the chromatin thread with the nu- 
cleoli of this Stage, e) Düring late spireme stages the nucleoli contract 
