IOWA ACADEMY OF SCIENCES. 
71 
not acid fast. Spores formed in abundance in twenty-four 
hours or less, polar in position rarely median, do not dis- 
tend mother cell, from 1.4-1. 8 microns long .6 microns 
broad, readily stained with double stain. These spores are 
very resistant to dessication and to heat. 
CULTURAL CHARACTERS. 
Dung Agar Plate Cultures. — Colonies amoeboid, in twenty- 
four hours, -A mm. in diameter, flat, thin, smooth, colorless 
to light gray. Under low power of the microscope colo- 
nies may be seen to consist of twisted threads of bacteria, 
edge of colonies rather indeflnite, slightly resembling B. 
mycoides, colony soft. Deep colonies similar but better 
defined. Very often under favorable conditions a few 
hours were sufficient to cover the plate with a uniform 
layer of the organisms. Sames notes this character in 
particular. “ Die Platte bei 62^ oft schon nach 6 Stunden 
uberwuchhern und zu dieser Zeit Sporen enthalten.” 
Nutrient Agar Plate. — Growth resembling that on young 
agar, but not nearly so luxuriant. 
Gelatine. — Gelatine is not peptonized. 
Milk. — No change perceptible, though mounts showed 
the organism to be present in numbers. Growth was not 
as luxuriant as noted by Sames. 
Litmus Lactose Agar Stab. — At the end of twenty-four 
hours very faint acid tinge. In forty-eight hours lower 
half of tube partially decolorized, upper half showing 
decided increase in alkalinity. Growth almost exclusively 
on the surface, thin, colorless. Spore production particu- 
larly abundant. 
Potato. — Very little or no growth. Mounts made from 
the surface of the potato showed the organism to be present 
in comparatively small numbers. 
Bouillon. — Uniform clouding in twenty-four hours, in 
forty-eight hours, a sediment. 
Agar Stab. — Surface growth as in plates. Practically no 
growth along the deeper portion of a needle track. 
