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IOWA ACADEMY OF SCIENCES. 
Dunham^ s Solution, — Little growth. Indol faint. 
Physiological Characters. Dessication. — The spores 
are capable of withstanding drying for an indefinite period. 
Temperature Optimum. — About 60^ C. Minimum about 
40^ C. Maximum about 70° C. 
Relation to Oxygen. — The organism is probably a strict 
8erobe, though a very slight development takes place in glu- 
cose agar stabs prepared according to Wright’s method. 
Fig. 3. Curve showing relation of growth to acidity or alkalinity of the medium. 
Pigment. — No pigment is produced. 
Gas Production. — No gas is produced in dextrose, lactose 
or saccharose media. 
Relation of Growth to Acidity, Alkalinity of Medium.— 
Bouillon tubes each containing 10 c.c. of Bouillon, exactly 
neutral to phenol-phthalein were treated with quantities of 
normal hydrochloric acid and sodium hydrate varying from 
^ c.c. to .3 c.c. The above table shows the results in the 
