IOWA ACADEMY OF SCIENCES. 
73 
form of a curve. The amount of growth in the tube con- 
taining the most growth was used as a standard, fixed 
arbitrarily at 10, and the remainder was scaled carefully 
with this as a basis . 
Indol Negative or very weak. Odor very 
little if any. 
Various media were prepared in order to determine the 
forms in which nitrogen and carbon are available to the 
organism as food. As a basis the Stickstoffreie “Min- 
eralische Nahrlosung” of Meyer was used. 
KH2P04 
Ig 
CaClg . . . 
Ig 
MgS 04 - 7 H 20 
3 g 
NaCl 
Ig 
FeaCle 
01 
HgO 
lOOOg 
To this solution was added various sources of nitrogen 
and carbon, as is shown, with results, in the following 
table: 
Number. 
Source of Nitrogen. 
Source of Carbon . 
Growth. 
1 
1 per cent Asparagin 
1 per cent Asparagin 
None. 
2 
1 per cent Asparagin 
1 per cent Glycerin 
None. 
3 
.05 per cent Asparagin 
1 per cent filter paper 
None. 
4 
1 per cent Urea 
1 per cent peptone 
Good . 
5 
1 per cent peptone 
1 per cent dextrose 
Good. 
6 
1 per cent peptone 
1 per cent dextrose 
Good . 
7 
1 per cent peptone 
1 per cent lactose 
Good. 
8 
1 per cent peptone 
1 per cent saccharose 
Good. 
It is probable that this organism is incapable of assimil- 
ating its nitrogen from any less complex compound than 
the peptones. 
Mikroscopisches Practlcum II. 15. 
