154 
IOWA ACADEMY OF SCIENCES. 
Several other species were also identified, but in some 
cases the identification is doubtful. Of these one is a new 
species of Sarcina occurring in capsules and of a canary 
color. Of the rod -shaped organisms are Bacterium n. sp. 
color oleaginous, Bacterium near B. Havaniensis, rose pink, 
and a Bacterium of lemon yellow color. These colors are 
described as they appeared on nutrient agar. 
Biological and morphological characters were determined. 
Under the latter head a study was made of the form and 
arrangement of the organisms. On the different media, the 
size, staining powers, motility, spores, and such special 
characters as capsules, involution forms, etc., were studied 
under the one-twelfth Homogeneous oil immersion lens. 
The biological characters were determined from their 
growth in the following media: gelatin plate, agar plate, 
milk, litmus milk, blood serum, gelatin stab, agar stab, 
agar slant, potato streak, lactose bouillon, glucose bouillon, 
saccharose bouillon, anaerobic glucose agar, Dunham^s 
solution, litmus agar. In all cases duplicate cultures 
were made. 
TABLE SHOWING RESULTS OP WELL AT AMES FOR ONE WEEK. 
Place. 
Date 
Plated, 
1904. 
Agar. 
Lit. 
Agar. 
Gelatin. 
Temper- 
ature. 
Remarks. 
C. &N.-W., Ames... 
Nov. 29. 
5400 
4800 
900 
600 
No gas. 
C. & N. -W. , Ames. .. 
Nov. 30. 
380 
500 
560 
500 
No gas. 
C. & N.-W., Ames. .. 
Dec. 2 . . 
840 
1000 
Lique. 
Part, lique- 
480 
No indol. 
C. & N. -W. , Ames. . 
Dec. 3 . . 
750 
Lique. 
Lique. 
No indol. 
C. &N.-W., Ames... 
Dec. 6 .. 
280 
Total liq. 
Total liq. 
65° C. 
No indol. 
C. & N. - W. , Ames. . . 
Dec. 8 . . 
52 
'46 
166 
i40 
500 
No indol. 
C. & N.-W., Ames... 
Dec. 9 . . 
230 
170 
200 
180 
Part liq. 
250 
* 
Non- liq. 
♦Lit. agar incubated at 3734° 0. 
