IOWA ACADEMY OF SCIENCES. 
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KH 3 PO,, 1.00 g., CaCl,, 1.00 g., MgS0,+7H,0, 0.3 g., NaCl, 
0.1 g., Fe.Cle, .01 g., Water, 1000 g. The various solutions 
made from this and their effect upon the growth and color 
production of this organism are given below. 
Dextrose, 0.5 g., cane sugar, 0.5 g., glycerin, 0.5 g., M. 
nahrlosung, 100.00 g. A very slight membranous sediment 
was formed. 
Potassium nitrate, 1.0 g., dextrose, 1.0 g., M. nahrlosung, 
100 g. Slight growth but no color was produced. 
Potassium nitrate, 1.0 g., glycerin, 1.0 g., M. nahrlosung, 
100 g. Some growth, but no color. 
Potassium nitrate, 1.0 g., cane sugar, 0.5 g.,glycerin, 1.0 g., 
M. nahrlosung, 100 g. Decided growth, but no color. It 
seems that this organism is capable of using its nitrogen 
in the form of nitrates, but can not produce color. 
Ammonium chloride, 1.0 g., dextrose, 1.0 g., M. nahrlosung, 
100 g. Slight sediment, whitish, granular, no color. 
Ammonium chloride, 1.0 g., cane sugar, 0.5 g., glycerin, 
0.5 g., M. nahrlosung, 100 g. Some sediment, whitish 
granular, more than in the preceding. 
Ammonium tartrate, 1.0 g., glycerin, 1.0 g., cane sugar, 
0.5 g., M. nahrlosung, 100 g., cloudy, granulated, whitish, 
sediment, no color. 
Ammonium tartrate, 1.0 g., dextrose, 1.0 g., M. nahrlosung, 
100 g. Cloudy, granulated, white sediment, rather more 
than in the preceding. 
Asparagin, 0.2 g., MgS04 , 0.1 g., K 2 HPO 4 , 0.1 g. Good 
growth, and decided color produced on the surface. 
Asparagin, 1.0 g., K2HPO4, 0.1 g., MgS04, 0.1 g., glyc- 
erin, 2.0 g. Good growth, large amount of color on the 
suface. The medium below the surface had a decided 
green tinge. This is to be noted in a large number of 
solutions in which asparagin was used. 
Asparagin, 1.0 g., M. nahrlosung, 100 g. Slight purple 
scum, white sediment, decided green tinge. 
Asparagin, 1.0 g., dextrose, 3.0 g,, M. nahrlosung, 100 g. A 
heavy purple scum and membranous white growth 
