50 
Franz Schräder 
plate — five chromosomes ranged haphazard around a central group of 
five others which evince a more or less marked tendency to duster (fig. 13). 
Although it is thus not certain that each chromosome group as here seen 
is directly derived from the sinailar group in the parent plate, yet it is 
only fair to assume that such is the case. 
Second Spermalocyte. 
There is no interkinesis worthy of the name. True, the ten chromo- 
somes partially lose their staining power so as to appear somewhat faint, 
but there is no truly diffuse stage, and the outline of the chromosomes 
remains fairly distinct (fig. 14 and 35). The staining power is regained 
preparatory to the second division. 
This division is decidedly unorthodox in its nature, but my preparations 
leave me no other interpretation than the one here given. In polar views 
the first Step appears to be initiated by a disarrangement of the plate 
as it has been described. Side views show that there is a Separation into 
two groups which begin to approach opposite poles. One of these groups 
is generally more massed than the other, but in favorable views both are 
seen to consist of five chromosomes (fig. 16). These counts become un- 
mistakable as the two groups progress toward their respective poles 
(fig. 17, 18 and 37). 
The arrangement in these two groups of chromosomes during division 
is, I think, a feature to be especially noted. The group showing the 
chromosomes massed often has a circular form, with the chromosomes 
touching end to end. In other cases, this massing may be more marked, 
so that a dense clump is formed. The sister group has a distinctly more 
scattered arrangement, and its chromosomes tend to straggle out or lag 
on their way to the pole (fig. 16 and 36). This trait is shown to a marked 
degree in P. nipae, and often practically every cell in a cyst shows such 
a characteristic division figure (fig. 45). 
This division plainly effects reduction — although it does so in ano- 
malous fashion. IVhat relation each of the separating groups bears to 
the groups in the first spermatocyte plate, it is difficult to say. The hypo- 
thesis which presents itself at once is that the massed group of five chromo- 
somes in the second division is directly derived from the aggregation of 
chromosomes which show such a similar behavior in the first division 
and can in tum be traced back to the dense chromatin of the resting 
stages. Subsequent behavior of the now separated groups lends some 
Support to such an hypothesis. 
