442 
Chas. AV. Aletz and Jose F. Xonidez 
is from a slightly later Stage in whicli tlie pairedassociation is so intiinate that all trace 
of duality is gone, and only five chromosomes can be detected. 
“The staining capacity of the chromatin is greatly decreased at this time and thc 
chromosomes appear less bulk}' than before. Xeedless to say, such figures are difficult 
to analyse, but a careful study has convinced us that the process is as described — that 
homologous members join in early telophase and effect an intimate union side by side. 
This conclusion is based not only on the duality of the telophase chromosomes, but on 
the fact that they are haploid in number (5) instead of diploid (10). The cells are small, 
affording plenty of examples of uncut nuclei, and in no case have \ve been able to find 
one in which the chromatic bodies approached the diploid number. Indeed, we found 
no clear case in which more than five were present. 
“Probably the density of the stain or degree of extraction has a good deal to do 
with the appearance or non-appearance of the duality in these telophase nuclei, but 
tliere can be no doubt that the union is very intimate. In this relation the chromosomes 
pass (fig. 9) into the succeeding stage in which they lose their staining capacity to a 
much greater extent, as they enter the growth period. A careful scrutiny of the late 
telophase nuclei reveals very little indication of a spimiing-out process or a net-work 
formation, except that due to the linin. The chromosomes appear simply to fade out 
through loss of color, while retaining, approximately, their form and position (fig. 9). 
“It is probable that the above account should not be restricted to the final spermato- 
gonia, but should apply to all of the spermatogonial telophases. The evidence points 
consistently in that direction (Metz ’16), but we have not been able to make sure of 
the point in the species under consideration.” 
In A. notatus the telophase phenomena seem to agree in all essentials 
with those of Ä. sericeus, and we are conficlent that the above description 
applies in the present case, except that the chromosoine number is different. 
This view is snpported also by the succeeding stages, represented in figs. 15 
and 16. As the chromatin begins to take the stain, after passing tlu-ough the 
telophase, it appears in the form of irregulär, grannlated bands or flocculent 
threads. IVith f urther condensation these passdirectly into the seven bivalent 
chromosomes of stage b, which inarks the beginning of the growth period. 
The latter changes niay now be considered in greater detail. 
Stage a and the early growth period. 
Following the final spermatogonial telophase there is a very brief 
period during which relatively little chromatin is visible in the nuclens, 
as indicated by fig. 14. This stage corresponds to stage a of A. sericetis, 
and like the latter, is characterized by the appearance of a small nucleolus 
(fig. 14). It agrees also in that the nuclei of this period, together with 
those of the telophase just preceding. are the smallest to be found in the 
testis and cannot be confused with those of any other stage. 
As noted in the case of A. sericeus, onr stage a appareutly corresponds 
to Davis’ (’08) stage a in the Orthoptera and Wilson’s (’12) stage a in 
