802 
PROCEEDINGS OF SECTION I. 
Those who are acquainted with the conditions of bush life — particularly 
the bush life of North-western Queensland — will, I would fain hope, 
look leniently on the many shortcomings of the little piece of 
experimental work I have attempted in this direction. 
Casting about for a suitable animal, it occurred to me that the 
goat enjoys a reputation for resistance to tubercular infection, and is 
moreover an animal easily available for experimental purposes. 
Accordingly, with a view of testing, in the first instance, the claims 
of the goat to resistance, I, in November, 1893, injected some tuber- 
cular sputum into the cellular tissue of the abdomen of two goats, 
each goat receiving about half an ounce. Three weeks later I repeated 
the operation, using on this occasion the sputum from a case of acute 
pulmonary and laryngeal phthisis, in 'which the bacilli were excep- 
tionally abundant. The two goats thus doubly inoculated were kept 
for purposes of comparison with four others of the same size, sex, 
and age. Ulceration took place at the points of inoculation in both 
goats. The inguinal glands became much enlarged, but did not 
suppurate, and gradually subsided. Otherwise the goats appeared in 
no way affected. They remained, and remain at the present time, 
more than a year after the inoculations, as well and healthy as the 
four control animals kept with them. The average temperature of 
healthy goats was found to be a shade over 102 degrees Fahr. Of 
twenty-six observations on the inoculated goats the highest tempera- 
ture recorded was 103 degrees Fahr., and the lowest 101*6 degrees 
Fahr. In May last (six months after the inoculations) *01 cm. 
of Koch’s tuberculme was injected on three several occasions into 
each of the inoculated goats, but on no occasion did any rise in 
temperature follow. From these facts I concluded that the goats’ 
reputation for resistance to tuberculosis was not altogether without 
foundation, for the same kind of inoculations practised on susceptible 
animals — e.g., guinea-pigs — almost invariably result in general infection, 
and death in a comparatively short time. 
So much being established, I endeavoured to ascertain if 
by frequently injecting tbe toxine of the tubercle bacillus (in the 
shape of Koch’s tuberculine) into a healthy goat I could so far bring 
about in its system such an increased development of tbe neutralising 
principle (tubercle antitoxine) as to make the serum sufficient as a 
curative agent when injected into animals suffering from tuberculosis. 
To this end six adult male guinea-pigs were on the 22nd duly, 
1894, inoculated with sputum from a case of rather chronic phthisis, 
in which the specific bacilli were present in moderate numbers. The 
operation was repeated four days later, great care being taken to 
prevent the escape of any of the sputum. Three of these guinea- 
pigs were placed in one cage for experiment, and the remainder in a 
second cage for control. The difficulty of obtaining these animals was 
the reason for employing so small a number. 
On 27th July a healthy goat was for the first time injected with 
*01 of Koch’s tuberculine. Thirty-six hours afterwards blood was 
taken from the jugular vein of this goat; and on the following day, 
when the serum had separated, 1 cm. of it was injected into the 
cellular tissue of the back of each of the three experimental guinea- 
pigs. 
