SERUM THERAI a EUTICS IX RESPECT TO TUBERCULOSIS. 803 
This process of injecting tuberculine into the goat, and subse- 
quently injecting the goat’s serum into the three experimental guinea- 
pigs, was repeated as under stated, it being understood that the 
bleeding of the goat was effected at varying periods from twenty-four 
to forty-eight hours or more after each injection of tuberculine. 
Goat Injected with Tuberculine. 
Experimental Guinea Pigs 
Injected witli Goat’s Serum. 
27th Julv 
*01 cm. 
29th July 
•1 cm. 
29th „ ... 
'01 
3rd August... 
■1 „ 
1st August... 
*02 
5? 
5th „ 
•1 „ 
3rd „ ... 
•1 
10th ,, 
•1 „ 
8th „ ... 
'1 
>> 
14th „ ... 
1 „ 
12th „ ... 
'2 
? J 
20th „ ... 
■1 „ 
18th „ ... 
'2 
27th „ 
•1 „ 
23rd „ ... 
*1 
4th Sept. ... 
•3 „ 
30th ,, 
•1 
17th „ ... 
•2 „ 
2nd Sept. ... 
*1 
>5 
26th „ ... 
•2 „ 
10th „ 
1 
13th October 
•2 „ 
24th „ 
•1 
j > 
19th „ ... 
•2 „ 
17th October 
•1 
?? 
On 9th October one of the control guinea-pigs died, this being 
the seventy-ninth day from the first and seventy-third from the second 
inoculation. Post-mortem showed the usual signs of experimental 
tuberculosis — enormous infiltration of liver, spleen, and lymphatic 
glands wfith tubercular deposit. 
On 18th October a second of the control guinea-pigs died. Post- 
mortem showed much the same condition as the first — enormous 
tubercular infiltration of liver, spleen, and lymphatic glands. 
The weather had now become extremely hot, and the limited 
means at my disposal made it extremely difficult to obtain and 
preserve the goat’s blood in a sterile condition during the separation 
of the serum, and to add any antiseptics would obviously vitiate the 
experiment. In spite of much care and the rejection of many tubes 
of serum, a contaminated specimen was accidentally employed at the 
last injection (on 19th October), resulting in the death of one of the 
experimental guinea-pigs two days later. At the post-mortem this 
guinea-pig presented a very marked contrast to the two control 
animals already referred to. It was fat and heavy. At the site of 
the last injection the cellular tissue was infiltrated with a quantity of 
dark sanguineo-serous fluid which was found in a cover-glass prepara- 
tion to swarm w ith the Bacillus septiccemice hcemorrhagicce. The liver, 
spleen, kidneys, lungs, and lymphatic glauds were perfectly free from 
any sign of tubercular deposit. 
The absolute freedom of this guinea-pig from tuberculosis seemed 
highly encouraging, especially in view of the fact that two out of the 
three control animals (untreated with serum injections) had already 
perished — a mass of generalised tuberculosis. 
But the sequel was less satisfactory. It should be mentioned 
that on account of the accident to this guinea-pig, and the extreme 
difficulty of preserving the serum, the injections were now discon- 
tinued. 
On 1st November one of the experimental guinea-pigs died of 
tuberculosis, the spleen being the organ chiefly affected. 
