CHEMICAL ABSTRACTS 
STUDIES ON YEAST V- IS BIOS A SINGLE 
SUBSTANCE 
ELLIS I. FULMER AND V. E. NELSON 
Abstract 
In previous communication (Journal of Biological Chemistry, 
March, 1922) Fulmer and Nelson showed that the water extract 
of alfalfa is much richer in the yeast growth stimulant, Bois, than 
is the 95 per cent alcoholic extract of the same material. In the 
work here described two extracts were prepared as follows from 
alfalfa which had been previously extracted with ether. Ex- 
tract A was an extract by long extraction with absolute alcohol. 
Extract B was an extract prepared by long extraction of the 
absolute-alcohol-extracted material with water. Both extracts 
showed optimum concentrations for maximum stimulation and 
were about equally potent. Combinations of the two extracts 
were much more potent than the optimum concentration of either 
alone. Detailed studies are being made of the properties of the 
two extracts. Bois is not a single substance but is composed of 
at least two materials. Bois A is soluble in absolute alcohol and 
in water. Bois B is insoluble in absolute alcohol and is soluble in 
water. 
Iowa State College. 
