3 
CRIUOIIB . 
At all times these are to be placed directly in full strength 
95$ alcohol. As you get them from the dredge, drop them in a deep enough 
pan and flood with the alcohol immediately. This enables you to get at 
them, before they begin to go to pieces, as they are very prone to do. When 
they are dead it is a simple matter to bottle them. 
WORMS (Folychaetes ) . 
For larger worms (over 25 mm. long): Clear of debris. Place in 
a dish sufficiently large to allow for complete expansion of the animal. 
Cover with clean sea water. Add slowly weak (about 5 $) formalin until the 
animal is dead. Transfer to weak (about 4-5$) formalin. 
For smaller worms (less than 25 mm. long): Use the same procedure 
as above, but use slow alcohol instead of slow formalin. Transfer to 70$ 
alcohol. 
Flat worms may be dropped in 70$ alcohol, or, better, formalin. 
They may be flattened under light pressure. 
SPALL AWBIAL3 1R0M SPOUSES . 
Sponges often afford protection to many small crustaceans, marine 
worms, etc., so if sponges be torn apart and rinsed in water many of these 
small creatures will become dislodged and may be collected from, the water. 
A portion of each sponge collected or a whole small one should be saved 
for sponge specimens. These are best put directly into full strength 
(about 95$) alcohol, poured the next day and refilled with 95$ and again 
on the third day. This is done with sponges because they are naturally so 
full of water that the alcohol is too quickly diluted otherwise. 
BOTTOM SAMPLES. 
The bottles are be 3 t only half filled with botto \ material and 
the bottle filled with 95$ alcohol and shaken up the next day to properly 
distribute the alcohol through the material. If bottle is more than, half 
full, the alcohol will have to be carefully poured off the next day without losing 
any of the finer sediments, and replenished with fresh alcohol. 
