250 MK. NEWPORT ON THE IMPREGNATION OF THE OVUM IN THE AMPHIBIA 
Which had been dipped into fluid only once for the whole, the time occupied in 
touching them being about five seconds. Each of these eggs became fertilized, an 
segmentation commenced in two of them in exactly /re hours, and m the remaining 
foL in frotn three to four minutes later. The period at which segmentation com- 
menced having been very carefully watched, and the whole of the eggs having been 
submitted, during the interval, to the same physical conditions of heat, light, quan i j 
of water and degree of aeration, the result, in regard to the difference of time at 
which they began to change, seemed to be accounted for only on the assumption 
that it was due to a greater quantity of influence applied to the first two than to the 
others ; and this opinion has since been more fully borne out by other experiments. 
The fluid at the time it was employed had been tliirty-five minutes mixed with 
water On the fifth day each of these eggs was producing an emiryo. 
Three eg-s were placed in separate cells, and each egg was touched once with the 
pin-head loaded only once for the three, the fluid employed having been twenty-three 
minutes mixed with water. After four hours and forty mmutes two of these eggs wei e 
undergoing segmentation, and on the fifth day both were producing embryos. The 
third egg was not impregnated. j i 
Twenty-two eggs, in a large cell, were each touched once with the pin-head loaded 
for each egg when the fluid had been one hour and forty -seven minutes mixed Mith 
water. Some of the eggs in this experiment were touched on the white surface 
others on the dark, and others at the side, or about midway between the white an 
dark surfaces. . , i ^ 
In the same cell with these eggs, but at a little distance from them, were placed 
eighteen other eggs, which were not touched with the pin-head, but wei-e simp y 
allowed to remain in the same cells with the twenty-two eggs experimented on, and 
the cell was then filled with pure water. At the end of four hours and fifty minutes 
the chamber had been formed, and segmentation had commenced in each ot the 
twenty-two eggs experimented on. But in addition to these there were also two ot 
the eighteen g which had not been experimented on undergoing segmentation. 
These during the expansion of their envelopes, had come into contact with some of 
the fecundated eggs, and had also become fecundated by some of the spermatozoa 
which had been supplied to them. Five days afterwards the whole of these twenty- 
four eogs were producing embryos 1 so that not only had the quantity of spermatozoa 
employed been fully suflicient to fecundate the eggs actually touched, but also others 
present in the same water with them. 
In another experiment, made before the preceding, but with some of the same brooc 
of eggs and same sample of fluid, I placed five eggs in a cell, and touched each once, 
very lightly, with the pin’s head loaded once only for the whole. This was when the 
fluid had been only eighteen minutes mixed with water. The object of this experi- 
ment was to learn whether there is any difference in the result when the eggs are 
touched at different parts of their surface. Accordingly the trial was made by touch- 
