432 ME. J. B. LAWES, DE. aiLBEET, AND DE. PUGH ON 
Page 
B. The Mineral Constituents added to the prepared soil 472 
C. The DistiUed AVater 473 
; D. The Pots used to receive the Soil, Ash, Plant, &c. 473—474 
E. Einal preparation of the Sod, Ash, and Pot, for the Plant 474 
E. The Seeds taken for Experiment 474 — 475 
G. The Atmosphere supplied to the Plants 475 — 476 
H. The Apparatus used to enclose the Plants, and to supply them with Air, AVater, 
Carbonic acid, &c ; '... 476 — 478 
I. Use of the Apparatus 478 — 480 
J. The supply of Carbonic acid to the Plants 480 — 481 
K. Advantages of the Apparatus adopted 481 — 483 
L. Adaptation for healthy growth of the conditions of experiment adopted 483 — 484 
Section II . — Other Conditions of Experiment requiring collateral Investigation -. — 
Enmueration of 484 
A. General considerations in regard to the possible influence of Ozone on the 
supply of combined Nitrogen to growing Plants 484 — 486 
B. Composition of the Gas in Plants 486 — 495 
C. Experiments on the action of Ozonized air on decomposing Organic Matter, and 
porous and alkaline substances 495 — 497 
D. Evolution of free Nitrogen in the decomposition of Nitrogenous organic 
matter 497 — 508 
E. Experiments on the action of the oxidizing and reducing forces, as manifested 
in the decomposition of Nitrogenous organic matter 509 — 515 
E. The mutual relations of Gaseous Nitrogen, and the Nascent Hydrogen evolved 
during the decomposition of organic matter 515 — 516 
Summary Statement of the Mesults of the consideration of the conditions required, or involved, in 
Experiments on the question of the assimilation of free Nitrogen hy Flants 516—517 
Section III . — Conditions of growth u/nder which assimilation of Free Nitrogen hy Plants is most 
likely to take place. Eirect Experiments upon the (Question under various cir- Z 
cumstances of growth : — 
A. General consideration of conditions of gTowth 517 — 520 
B. Direct experiments on the question of the assimilation of free Nitrogen by plants 520 — 540 
I. Experiments in which the plants had no other supply of combined Nitrogen 
than that contained in the seed sown 521 — 529 
II. Experiments in which the plants had a known supply of combined Nitro- 
gen, beyond that contained in the original seed 529 — 538 
Eelations of the plants grown with an extraneous supply of combined nitrogen, 
to those grown without it 538 — 539 
Conclusions 539 — 540 
Summary of the Pesults of the whole Inquiry 540 — 541 
Appendix. 
A. Preparation of solutions for manuring the plants, dates of application, and 
quantities applied 542: — 543 
B. Taking up the plants, preparation for analysis, methods of analysis, &c 543 — 547 
C. Abstract of the Eecords of Growth of the Plants. 
I. Plants grown in 1857 548 — 558 
II. Plants grown in 1858 558—577 
Plates Nil. XIII. XIV. and XV. 
