DE. ON STJGAE POEMATION IN THE LIVEE. 
605 
It then yielded 3-37 per cent, of amyloid substance. In No. 5 the liver was left in 
the animal for ten minutes and then removed, but not examined for the space of two 
hom's. It then yielded 3-51 per cent, of amyloid substance. In No. 6 an error occuiTed 
in the steps of the analysis, rendering it valueless. 
The usual allowance for a dog in my laboratory is one of the ordinary penny bundles 
of tripe diem. In examples Nos. 7 and 8 an extra allowance was given, to see if it 
occasioned any difference. To No. 7 was given two bundles of tripe daily for four days 
prior to its death. No. 8 was allowed its full tether, and the quantity of tripe it 
devoured was truly enormous ; three bundles one day, four another, five the third, and 
two and a half the day it was killed. The figures yielded do not disclose any significant 
variation from the ordinary condition. 
To ascertain the effect of a vegetable diet on the liver, I submitted five dogs to a 
course of food for several days previous to death, consisting of barley meal and potatoes, 
or, where this was refused, to bread and potatoes. The following are the results 
obtained : — 
Tlie lAver of the Bog under a Biet of Vegetable Food. 
"Weight of dog. 
lbs. oz. 
Absolute weight 
of liver, 
oz. 
Eelative weight of 
liver to animal. 
No. 12 . 
... 17 
8 
1 to 14i 
No. 13 . 
... 11 
8 
1 to 14i 
No. 14 . 
... 15 
8 
Ilf 
1 to 21 
No. 15 . 
... 18 10 
28 
1 to 101 
No. 16 . 
... 17 
5 
121- 
1 to 22^ 
Total 
... 80 
7 
83f 
1 to 15 
Taking the average of these five instances as a sample of the effect of a vegetable diet 
on the liver of tlie dog, it appears that the organ rather more than equals in ounces the 
number of pounds the animal weighs. It will be remembered that, after an animal 
diet, the average given was rather under the half ounce to the pound. The facts as to 
the amount of amyloid substance stand thus; — 
No analyses were made of the livers of the dogs Nos. 12 and 13, but the quantity of 
amyloid substance was unusually large. It was whilst examining these livers for the 
determination of sugar that I was first led to notice the effect of a vegetable diet that I 
am now describing. 
Amount of Amgloid Substance in the Liver of the Bog after a Biet of Vegetable Food. 
per cent. 
Example No. 14 9-87 * 
Example No. 15 25 '30 
Example No. 16 16’50 
Average amount of amyloid substance yielded by the above three analyses 17’23 per cent. 
* This liver not examined till one and a half hour after death. 
