16 
and 2 growing vigorously, with fully developed roots and leaves ; in 
No. 3, at the end of four mouths the trees were living, but feebly, and 
the roots showed signs of decay. 
In No. 4 the trees had grown somewhat better and had a brighter 
color, but the roots were knotty. 
No. 5 showed poorly, leaves smaller, roots quite knotty, and one tree 
dead. 
The same results followed using the Weeping Willow as the test 
plant. 
The inferences are : That the Anguillula is destroyed by a heat of 
212°; that healthy trees set in infected soil soon are invaded by the 
tree Anguillula in the soil ; that soil taken from the depth of 2 or more 
feet below the surface is comparatively free from the worms, and that 
clay subsoil is less infected than the sand. 
Another series of experiments testing the effect of various chemicals, 
fertilizers, and insecticides was tried, using four sterilized pots in each 
test, the pots each containing cubic foot of infected sandy soil, and 
the chemical, at the rate of T ^ro of the weight of the soil (24 grains), thor- 
oughly mixed or dissolved. In each pot was planted a seedling peach 
and four cow-peas. 
No. G. Tobacco (lust. 
7. Tobacco dust with 24 grains sulphate potash. 
8. Tobacco dust with 24 grains sulphide potash. 
9. Tobacco dust with 24 grains sulphite potash. 
10. Tobacco dust with 24 grains muriate potash. 
11. Tobacco dust with 24 grains hyposulphite soda. 
12. Tobacco dust with 24 grains sulphate iron. 
13. Tobacco dust with 24 grains caustic lime. 
14. Tobacco dust with 24 grains unleached ashes. 
15. Tobacco dust with 24 grains sulphur. 
1G. Bisulphide carbon. 
17. Sulphate potash. 
18. Muriato potash. 
19. Unleaclied ashes. 
20. Caustic lime. 
These experiments were also repeated in the nursery and open field 
on small peach trees, using G02 grains to each tree, equivalent to poVo 
part by weight of the soil. (Note 6.) 
In the pots the results in Nos. G, 7, 10, 13, and 14 were very encour- 
aging; the peas grew to maturity, with good color and very few en- 
larged roots. Nos. 8, 9, 11, and 15 made a very poor growth, and died 
soon after the third leaf. No. 12 died immediately after sprouting, as 
did No. 1G. Nos. 17, 18, and 19 grew nearly as well as Nos. G and 7 ; 
No. 20 made a fine growth, with very few enlarged roots. The peach 
trees died soon in Nos. 8, 9, 11, 12, 15, and 1G, made a fair growth in Nos. 
G, 7, 10, 13, and 14, were killed at once in No. 1G, and grew the best in 
Nos. 17, 18, 19, and 20. 
In the field Nos. 11, 1G, and 8 appeared to at once kill the trees. Nos. 
