136 
T. C. Kane, T. C. Barr, Jr., and G. E. Stratton 
Mountains, including Pisgah Ledge. We sampled three populations of 
vandykei and four populations each of bowlingi and pisgahensis ; thus 
differentiation between local populations within the same continuous 
upland can serve as a baseline for comparing taxa on isolated peaks. 
The isolates within the vandykei species group can be arranged in 
five subgroups, each bearing one of the five available trivial names of 
taxa assigned to the species group. Although all 12 isolates differ to a 
greater or lesser extent in minor morphological characters and will be 
the subject of a subsequent taxonomic paper (Barr, in preparation), we 
propose no new names in this paper. The subgroups are as follows. 
1) vandykei subgroup — (a) T. vandykei Jeannel (1931), Black and 
Great Craggy mountains and adjacent Blue Ridge, Yancey Co. and 
MacDowell Co., N.C.; three other isolates on (b) Camp Creek Bald, 
Greene Co., Tenn., and Madison Co., N.C.; (c) Big Bald, Unicoi Co., 
Tenn., and Yancey Co., N.C.; and (d) Unaka Mountain, Unicoi Co., 
Tenn., and Mitchell Co., N.C. 
2) bowlingi subgroup — one isolate, T. bowlingi Barr (1962), widespread 
in the eastern two-thirds of the Great Smoky Mountains National Park, 
Tenn. and N.C. 
3) tusquitee subgroup — (a) T. tusquitee Barr (1979), Tusquitee 
Bald, Macon Co., Clay Co., and Cherokee Co., N.C.; two other isolates 
on (b) Joanna Bald (Snowbird Mountains), Graham Co. and Cherokee 
Co., N.C.; and (c) Cheoah Bald, Graham Co. and Swain Co., N.C. 
4) haoe subgroup — one isolate, T. haoe Barr (1962), known only 
from Haoe Lead above Joyce Kilmer Memorial Forest, Unicoi Mountains, 
Graham Co., N.C. 
5) pisgahensis subgroup — (a) T. pisgahensis Barr (1979), widely 
distributed in the Great Balsam Mountains and their eastern arm, 
Pisgah Ledge, in Buncombe Co., Haywood Co., Jackson Co., and Transylvania 
Co., N.C.; two other isolates on (b) Sandymush Bald, Newfound 
Mountains, Haywood Co. and Madison Co., N.C., and (c) Whiteside 
Mountain, Jackson Co. and Macon Co., N.C. 
METHODS 
A total of 19 populations representing 11 of the 12 known isolates 
of the vandykei group were sampled during the summer in 1982, 1983, 
and 1984 for electrophoretic analysis. [We were unable to recollect the 
Joanna Bald population discovered in 1960 (Barr 1962), although other 
Trechus (Microtrechus) species belonging to the nebulosus group were 
found there in some abundance.] Beetles were collected by sifting forest- 
floor litter or moss from a hardware-cloth basket into a plastic dishpan, 
where they could be removed with an aspirator or by hand. The most 
