60 
Burr, Kuhajda, Dimmick, and Grady 
and young were preserved for subsequent examination. In most cases, 
mated pairs were returned to their nest sites if they were not guarding 
embryos or larvae. 
Aging. Age of some specimens in breeding condition was determined 
by counting the number of annuli on cross sections of pectoral spines as 
outlined by Clugston and Cooper (1960) and modified by Mayden and 
Burr (1981). Many specimens were assigned to age classes based on 
length-frequency analysis, although neither method of aging provided 
satisfactory results. Lengths of juveniles and adults are expressed in mm 
standard length (SL); lengths of larvae in mm total length (TL). 
Food. Diet was determined from examination of the contents of 
the stomach. 
Substrate. Size ranges in centimeters of certain substrate types 
mentioned herein are: sand, less than 0.3; pea or small gravel, 0. 3-3.0; 
medium gravel, 3-5; large gravel, 5-8; small rubble, 8-15; medium 
rubble, 15-22; large rubble, 23-30; and boulder, greater than 30. 
MATERIALS 
A virtually complete record of repository is given. Institutional 
acronyms are: ANSP, Academy of Natural Sciences of Philadelphia; 
DU, Duke University; CAS-SU, Stanford University (now at California 
Academy of Sciences); NCSM, North Carolina State Museum of Natural 
Sciences; SIUC, Southern Illinois Univeristy at Carbondale; UF, 
University of Florida, Florida State Museum; UMMZ, University of 
Michigan Museum of Zoology; UNCC, University of North Carolina at 
Charlotte; and USNM, National Museum of Natural History. Numbers 
of specimens (in parentheses) follow the catalog number or the acronym. 
A total of 326 specimens (70 collections) were examined. Within 
each drainage, collections are listed alphabetically by county. Collections 
from the same site are listed in chronological order. An asterisk 
preceding a locality denotes collections made by personnel of the North 
Carolina Wildlife Resources Commission (NCWRC). To our knowledge, 
these collections were not deposited in a recognized museum or university 
collection, but are reported in Bayless and Smith (1962) and Smith and 
Bayless (1964). 
Neuse River System. CRAVEN COUNTY: NCSM 9939(1) Neuse 
River near Streets Ferry, 8.7 airmi. NW New Bern, 28 January 1981. 
DURHAM COUNTY: NCSM 1930(1) Eno River at SR 1004 bridge, 3 
August 1961. GREENE COUNTY: *NCWRC(22) Contentnea Creek, 3 
mi. S Scuffleton at Edwards Bridge, 6 September 1960. JOHNSTON 
COUNTY: DU uncat. (3) Mill Creek, 1 mi. N Cox Mill, SR 1200 bridge, 
8 June 1961; NCSM 13838(1) Little River at SR 1002 bridge, 1 mi. N 
