194 
MR. NEWPORT ON THE IMPREGNATION OF 
made, and to detail them when considering the subject to which they more especially 
refer. 
No. 1. p.m.2^52®. — Eighty-three ova were passed into water, and the impregnating 
fluid added to them at the expiration of one minute. This was forty-three minutes 
after the fluid had been obtained and mixed with water ; but on examination with 
the microscope at this period, the spermatozoa contained in it were still very active. 
This long period was determined on with a view to the more effectually testing the 
susceptibility of the ovum, as it will be shown that the spermatozoa are less and less 
efficient in proportion to the length of time they have been mixed with water. Seg- 
mentation commenced in a very large proportion of the ova at the end of three hours 
andjifty-five minutes. On the 14th of April, the eighth day, thirty-two embryos had 
been formed. 
No. 2. p.M. 2'‘ 51“. — Ninety -two ova were passed into water, and at the expiration 
of two minutes impregnating fluid was added to the S2iV[\Q,forty-three minutes after it 
had been obtained. Segmentation commenced in these also at three hours and Jifty-five 
minutes, and took place in almost every ovum. On the eighth day there were forty- 
five embryos. 
No. 3. p.M. 2*^ 24 “. — One hundred and twenty-seven ova were immersed in water 
for three minutes, and then exposed and bathed with impregnating fluid during 
twenty seconds, water being immediately afterwards added to them. No segmentation 
had taken place at the end of four hours and three minutes, but it took place in many 
of the ova at a later period, the exact time having escaped my notice. The fluid 
employed had been mixed with water only seventeen minutes. On the eighth day 
there were thirty-three embryos. 
No. 4. p.M. 2'‘ 15“. — Eighty-one ova were exposed to the air on a dry surface for 
three minutes without having been in contact with water, and were then bathed with 
impregnating fluid during seconds and water immediately afterwards added to 
them. The fluid in this experiment had been obtained only eight minutes. Segmenta- 
tion commenced in several ova at four hours and Jive minutes, and on the eighth day 
there 'Nc.xe, fifty -three embryos. 
No. 5. p.M. 2^^ 25“. — One hundred and thirty-six ova were passed into water ior Jive 
minutes, and were then exposed and bathed with impregnating fluid for several seconds, 
and water immediately afterwards added to them. The fluid had been obtained 
twenty minutes. Segmentation occurred in one ovum at four hours and eight minutes, 
and in others quickly after. On the eighth day only ten embryos had been formed. 
No. 6. p.M. 2*' 17'". — One hundred and thirty-nine ova were exposed to the air, on 
a dry surface, for Jive minutes, and were then touched freely with fluid during Jive 
seconds, applied with a hair-pencil, and water was then quickly added to them. The 
fluid employed had been obtained twelve minutes. Segmentation took place in four 
hours and eleven minutes. On the eighth day there were thirty-seven embryos. 
No. 7* P-M. 2'* 21 ™. — Two hundred and Jive ova were retained in water ior fifteen 
