238 
MR. NEWPORT ON THE IMPREGNATION OF 
Table II. — Experiments with Media that act chemically or me- 
Tempera- 
0) 
ture at the 
time of the 
-5 c 
eS V 
No. of eggs 
Embryos at 
experiment. 
S.-C 
S g. 
Segmentation 
comenced in 
Time, pro- 
No. of ex- 
Date and hour of 
Nature of the 
cured aod 
periraents. 
experiment. 
eCj 
O. 
o 
B 
c 
ns 
s s 
eS .S 
hours. 
a 
_C 
*&. 
'O 
C 
8 
End of 
third 
stage. 
In days 
and hours. 
impregnating fluid. 
mixed with 
water. 
< 
w 
Vi 
1850. 
SetV. 1. 
Mturch 25. p.m. 1 40 
48 
46 
61 
54 
20 
4“ 12'> 
Semen and water. 
10“ 
2. 
p.M. 1 45 
48 
46 
61 
39 
2 
41 121“ 
Semen and water. 
15“ 
3. 
P.M. 1 50 
48 
46 
61 
47 
Not one. 
Not one. 
Semen and water. 
20“ 
4. 
P.M. 1 53 
48 
46 
61 
45 
3 
51 
Semen and water. 
23“ 
5. 
P.M. 2 21 
48 
46 
61 
59 
Not one. 
Semen and water. 
51“ 
6. 
P.M. 2 26 
48 
46 
61 
72 
Not one. 
Semen and water. 
56“ 
1. 
P.M. 2 16 
48 
46 
61 
44 
Not one. 
Semen and water. 
46“ 
8. 
P.M, 
48 
46 
61 
76 
Not one. 
Semen and water. 
10” 
Set%. 1. 
P.M. 2 22 
48 
46 
61 
76 
Not one. 
Semen and water. 
52“ 
2. 
P.M. 
48 
46 
61 
58 
Not one. 
Semen add water. 
12“ 
&^Q. 1. 
30. P.M. 4 45 
49 
48 
61-5 
45 
12 
Not one. 
Semen from testes. 
10“ 
2. 
P.M. 4 58 
49 
48 
61-5 
53 
4 
Not one. 
Semen from testes. 
23“ 
3. 
P.M. 4 52 
49 
48 
61-5 
122 
50 to 60 
Not one. 
Semen from testes. 
17“ 
4. 
P.M. 5 
49 
48 
61-5 
66 
2 
Not one. 
Semen from testes. 
25“ 
5. 
P.M. 4 55 
49 
48 
61-5 
54 
1 
Not one. 
Semen from testes. 
20“ 
6. 
P.M. 5 2 
49 
48 
61-5 
79 
Not one. 
Semen from testes. 
27“ 
7. 
P.M. 5 15 
49 
48 
61-5 
77 
43 
Not one. 
Semen from testes. 
40“ 
8. 
P.M. 5 9 
49 
48 
61-5 
r 18 to 20>‘ ] 
76 
Not one. 
Semen from testes. 
34“ 
Set?,. 3. 
P.M. 5 5 
49 
48 
at temp. J- 
[49 to 48° J 
108 
3 
Not one. 
Semen from testes. 
30“ 
4. 
P.M. 5 18 
49 
48 
18 to 20*' 
58 
4 
Not one. 
Semen from testes. 
43“ 
Set K. 1. 
April 3. P.M. 3 5 
60 
4h 
82 
12 partial 
2 
6 to 71 
Semen and water. 
Ih 12“ 
2. 
60 
25 
1 
1 
6 to 71 
Semen and water. 
3. 
P.M. 3 10 
60 
4^'' 
58 
1 partially 
Not one. 
Semen and water. 
Ih 17“ 
4. 
60 
69 
Not one. 
Semen and water. 
U 
5. 
P.M. 3 17 
60 
4h 13m 
62 
30 to 40 
23 
51 
Semen and water. 
Ih 24“ 
6. 
P.M. 3 31 
60 
4h ] 
74 
12 to 15 
12 
51 
Semen and water. 
U 38“ 
7. 
60 
4'' 14“ 
59 
13 
10 
51 
Semen and water. 
8. 
P.M. 3 37 
60 
4h 20” 
79 
25 
25 
51 
Semen and water. 
Ih 40m 
Set S. 5. 
P.M. 3 25 
60 
4» 5” 
61 
33 
11 
51 
Semen and water. 
Ih 34“ 
6. 
P.M. 3 22 
60 
4h 30n> 
70 
10 
2 
71 
Semen and water. 
Ih 31 “ 
7. 
P.M. 3 41 
60 
4h 24“ 
85 
74 
42 
61 
Semen and water. 
Ih 50“ 
8, 
P.M. 5 50 
60 
132 
Nnt niiR. 
4 hOUTH 
Set S. 9. 
6. PM. 1 50 
60 
4h 24” 
122 
3 
3 
71 
Semen and water. 
30“ 
10 . 
P.M. 1 40 
60 
4h 20” 
158 
8 or 10 
5 
71 
Semen and water. 
20“ 
11 . 
P.M. 1 47 
60 
4i 25“ 
71 
3 
3 
71 
Semen and water. 
27“ 
12. 
P.M. 1 44 
60 
4h 18 “ 
119 
12 
541 
Semen and water. 
24” 
Total number 
2634 
369 
176 
f in Sets P, 1 
1 q,ii,&s./ 
RECAPITULATION AND CONCLUSIONS. 
It may now be well to recapitulate briefly some of the facts and views derived 
from the foregoing observations and experiments. First, then, the germinal vesicle 
disappears in the Amphibia before impregnation ; and before, or at the time of the 
bursting of the ovisac, and extrusion of the egg from the ovary into the cavity of the 
