THE OVUM IN THE AMPHIBIA. 
239 
chanically on the Spermatozoon or the Ovum in Impregnation. 
Material employed. 
Period of experiment. 
Operating 
mechanically. 
Operating 
chemically. 
Quantity per 
ounce of 
water. 
Interval 
before 
fluid. 
Interval 
after 
fluid. 
Whole 
period. 
Solut. caust. pot. 
Solut. caust. pot. 
Solut. caust. pot. 
Solut. caust. pot. 
Solut. nitr. pot. 
Solut. nitr. pot. 
Acetic acid. 
Acetic acid. 
9j to 3 ]. 
9j to sj. 
9j to §j. 
9j to §j. 
9j to 3j. 
15 sec. 
5 sec. 
10 sec. 
jm 
40 sec. 
30 sec. 
30 sec. 
30 sec. 
30 sec. 
20 sec. 
20 sec. 
1” 
1“ 
35 sec. 
45 sec. 
45 sec. 
62 sec. 
50 sec. 
45 sec. 
30 sec. 
45 sec. 
1"> 
45 sec. 
15 sec. 
20 sec. 
15 sec. 
12 sec. 
5 sec. 
6 sec. 
5 sec. 
5j to §j. 
Si to §j. 
5 sec. 
5 sec. 
15 sec. 
15 sec. 
Solut. gum-arab. 
Solut. gum-arab. 
Solut. caust. pot. 
Solut. caust. pot. 
Solut. caust. pot. 
Solut. caust. pot. 
Solut. caust. pot. 
Solut. caust. pot. 
Solut. nitr. pot. 
Acetic acid dilut. 
gr.viij to 5/. 
9y to 
Weak. 
Strong. 
Weak. 
Strong, 
gr. viij to 5j. 
5 sec. 
2 sec. 
2 sec. 
1 sec. 
2 sec. 
1 sec. 
1 sec. 
1 sec. 
Solut. gum-arab. 
Solut. gum-arab. 
1 sec. 
1 sec. 
Solut. caust. pot. 
Solut. caust. pot. 
Solut. caust. pot. 
Solut. caust. pot. 
Strong. 
Strong. 
Strong. 
Strong. 
1 sec. 
2 sec. 
1 sec. 
1 sec. 
Solut. nitr. pot. 
Solut. nitr. pot. 
Solut. nitr. pot. 
Strong. 
Strong. 
Strong. 
1 sec. 
3 sec. 
20 sec. 
25 sec. 
20 sec. 
20 sec. 
20 sec. 
3 sec. 
Solut. gum-arab. 
Solut. gum-arab. 
2 sec. 
2 sec. 
Solut. gum-arab. 
Solut. of starch. 
Solut. of starch. 
Solut. of starch. 
3 sec. 
10 sec. 
10 sec. 
2 sec. 
16“ 
Remarks. 
{ No. 1 to 8 of this set removed after fifty minutes to tempe- 
rature 61°Fa.hr. These ova were passed on a dry surface, 
and fluid applied freely with a pencil, and afterwards the 
potass washed oflF freely with water. 
Potass allowed to remain. 
Ditto, well bathed with fluid after potass. 
("Well bathed with solut. nitr. potass and water then added, 
\ the solution allowed to remain. 
J Impregnating fluid applied quickly and profusely, and water 
L then added. 
/Water was added quickly after the gum, but not sufficient 
\ to remove it ; one or two ova segmented. 
r No. 1 to 8 of this set also removed at end of nineteen hours 
•< to high temperature, 62° Fahr., No. 1 and 2 being 
[ thoroughly bathed with seminal fluid. 
Water added and solut. of potass not washed off. 
Nearly the whole at twenty hours spoiled. 
All spoiled in a few hours. 
Ova passed on a moist surface and well lathed with fluid. 
{ This was a thick solution of gum and applied with difficulty, 
but freely and quickly ; the eggs were then well bathed 
•with fluid and allowed to remain in a low, and slightly 
diminishing temperature. 
/ Ova touched lightly for an instant only with the fecun- 
\ dating fluid, and water afterwards added. 
/ Time of applying the potass was from two to three seconds ; 
\ the eggs were spoiling within twenty minutes. 
/ Made with fecundating fluid without solution of potass to 
\ test the above. 
/ These ova were passed on a dry surface and were then 
\ thoroughly bathed with seminal fluid and water. 
The fluid was applied after the solution. 
Impregnation must thus have occurred very quickly. 
/ Made to test the fecundatory fluid at two hours after mixture 
L with water. 
Not one egg segmented or one embryo found. 
/ Gum applied thickly and the ova then bathed with fecun- 
1 dating fluid. 
/ The ova passed into the starch, and a large quantity of fluid 
/ then added. 
Ova passed on a dry surface, starch applied with a pencil. 
Changed to fresh water after sixteen minutes. 
abdomen. It does not return to the centre of the yelk, nor escape to the surface, 
hut is lost much nearer to the latter than to the former position ; and its disappear- 
ance is the result of the endogenous development of cells in its interior. The egg is 
cast loose into the abdomen, and then consists only of the yelk mass in its vitelline 
membrane, and it is transferred to the mouth of the oviduct by the joint action of 
