62 DR. BENCE JONES ON A NEVE SUBSTANCE IN A CASE OF MOLLITIES OSSIUM. 
boiling and filtering whilst hot ; on cooling, the hydrated oxide will be precipitated 
from the filtered liquid, and it will again be redissolved by heat, whilst the albumen 
will remain on the filter. 
Conclusions. 
1 st. The ultimate analysis 
of this substance may be represented by C 48 , 
^385 ^ 6 ’ ^185 
or 
by C 40 , 
H3I5 Ng, 
O 45 ; accoi 
fding as protein 
is C 48 , H 37 , Ng, 
Oi 5 , or C 40 , 
^30? ^55 Ol 2 - 
B 33 ' ^6, Ojs ; 
or C 40 , Hgj.Nj, O 15 . 
1 st. 
2 nd. 
3rd. 
4th. 
5th. 
6 th. 7th. 
Reckoned. 
Reckoned. 
C 
51*68 
51*50 
52*25 
52*29 
52*16 
52*19 52*25 
52*10 
52*00 
H 
7-15 
7*09 
6*97 
7-17 
7*16 
6*97 
6*70 
6*85 
N 
15*24 
14*81 
15*17 
15*15 
O 
26*00 
25*99 
The sulphur and phosphorus in this substance, per cent., gave 
Sulphur =T36 TOO T03 per cent. 
Phosphorus . . . . = *20 ’19 per cent. 
Hence it is an oxide of albumen, and from the ultimate analysis, it is the hydrated 
deutoxide of albumen. 
2nd. In the above case of mollifies ossium 66*97 parts of this hydrated deutoxide 
of albumen were passing out of the body in every 1000 parts of the urine. Hence, 
therefore, there was as much of this peculiar albuminous substance in the urine as 
there is ordinary albumen in healthy blood. So far, then, as the albumen alone is 
concerned, each ounce of urine passed was equivalent to an ounce of blood lost. 
3rd. The peculiar characteristic of this hydrated deutoxide of albumen was its 
solubility in boiling water, and the precipitate with nitric acid being dissolved by 
heat and reformed when cold. By this reaction a similar substance in small quan- 
tity may be detected in pus and in the secretion from the vesiculae seminales. 
4th. This substance must again be looked for in acute cases of mollifies ossium. 
The reddening of the urine on the addition of nitric acid might perhaps lead to the 
rediscovery of it ; when found, the presence of chlorine in the urine, of which there 
was a suspicion in the above case, should be a special subject of investigation, as it 
may lead not only to the explanation of the formation of this substance, but to the 
comprehension of the nature of the disease which affects the bones. 
Lastly, I am much indebted to Dr. Watson and Dr. MacIntyre for enabling me 
to follow out this case, and also to Professor Fownes for the use of his laboratory at 
University College. 
