214 Inoculation for Leguminous Crops , 1905. 
earthenware pots, sixteen for each kind of soil. In four of each 
set peas were grown, the seed being previously “ inoculated ” 
in two of them and not inoculated in the other two : similarly, 
four other pots were sown with beans, four with spring tares, 
and four with red clover, the seed being inoculated in half the 
number and left untreated in the other half. 
The instructions given for inoculation of the seed were 
scrupulously observed, the seed being kept in the preparation 
for forty-five minutes and in the dark. The actual sowing 
took place with peas and beans on April 3, and with tares 
and clover on April 17, 1905. 
In the foregoing, the German method of Dr. Hiltner, was 
used. At the same time twenty-four zinc pots were used for a 
similar set o/ experiments with the American preparation of 
Mr. Moore, the crops sown being soy bean and melilotus, and 
the same three kinds of soil being used as before in each case, 
the seed being inoculated in one half and not in the other half. 
The dates of sowing were April 22, 1905, for the soy bean, and 
April 29 for melilotus. 
Simultaneously, plots in the open were sown with each 
variety of seed, this being “ treated ” in one case and “ untreated ” 
in the other. In the pot experiments twelve seeds were sown per 
pot for all the crops except melilotus, of which twenty-two seeds 
per pot were planted. In the open, 400 seeds of each kind 
were sown except with melilotus, when 1,222 seeds were used. 
In the case of the pots, the intention was to let the plants come 
up and then reduce them to six in number in each pot. 
The dates of appearance of the shoots were noted in each 
instance and a record of these duly kept. The peas and beans 
came up well in all three kinds of soil whether treated or 
untreated, the tares not quite so well, but fairly on the whole ; 
red clover, however, failed practically throughout, as did also 
melilotus and, to a large extent, soy bean. The blanks were, 
however, made up, where practicable, with plants that were 
taken from the similar plots grown in the open, and where 
germination was generally more successful. 
By May 25, peas and beans looked very well, and at first 
the treated seed appeared better, the foliage having a darker 
colour ; this difference, however, soon passed off. On June 8 
the plants were thinned to six per pot. The superfluous bean 
plants thus removed were washed and photographed. The 
photographs showed, however, practically no difference between 
the treated and untreated seed, and it was remarkable that 
plants in sterilised soil and showing no nodules on the roots 
had made such good growth. The bean plants from fertile 
soil were larger and stronger, but between the treated and 
untreated seed in this soil there was, again, nothing to 
