1882.] 
447 
[Kingsley. 
this is certainly not the case. At no time can these processes be 
seen to pass through either of the envelopes of the body and con- 
sequently they cannot serve as the points of attachment. They 
seem to me to be merely the remnants of the organs which in 
some ancestor were possibly for fixing the young Tunicate in a 
manner analogous to that presented by the antennae and cement 
glands of the Cirripedia, but which have lost this capacity and 
which have not entirely disappeared through disuse. 
After becoming attached the processes grow larger and longer 
and then are finally absorbed, the embryos passing through the 
condition shown in figs. 14-18. In the interior certain changes 
can be seen, but, owing to the opacity of the embryos, too indis- 
tinctly to enable one to recognize their character and significance. 
A central portion becomes darker than the rest and becomes 
folded on itself, but what organ in the adult each portion finally 
developes I was not able to ascertain. 
At this stage of my studies other forms in whose embryology I 
was much more interested presented themselves, and the jar con- 
taining the Ascidian embryo was set aside, the more willingly as 
I was tired of studying forms which for a week had shown no 
appreciable change. Three weeks after the stage shown in fig. 
17 was reached I again examined my embryos which were living 
in a jar, the water of which had not been changed in the time, 
and then many new features were observed. Fig. 18 represents 
one of these embryos. It was very difficult to obtain satisfactory 
views of the internal organs at this time, since the dust which had 
fallen into the jar had become attached to the young Ascidians ; 
still certain important features could be seen. This figure is a 
side view the atrial aperture being toward the observer. The 
branchial sac is now open and in connection with the outer world ; 
the longitudinal plications are faintly seen, but I was not able to 
see any of the branchial arches. The oesophagus is a straight 
cylindrical tube and the stomach is scarcely differentiated from it, 
though the line of demarcation between the stomach and intestine 
is very evident The intestine is short and small and is Hexed 
upon itself in the drawing, but this is the result of the position of * 
the specimens, the intestine in reality has but a slight curvature 
though the whole alimentary canal is flexed as in the adult. 
The eye still remains, marking the position of the ganglion of the 
