5 8 Newcomhe. — Cellulose- Enzymes . 
and the two contour bands lying next the cell-lumina remain 
brilliant for some hours after the intervening broader zones 
have become hyaline, while with the Aspergillus- ferment, the 
hyaline condition is first seen in the middle of the wall, and 
progresses toward the cell-lumina. It is somewhat remarkable 
though that when the wholly hyaline wall begins to disappear, 
it thins down from the borders toward the middle of the wall, 
so that a faint and thin middle lamella often persists for 
forty-eight hours after the beginning of the experiment. The 
walls of the aleurone-layer become hyaline in the same manner 
as with the Barley-ferment, that is, centrifugally from the 
cell-lumen to the middle of the wall. The action of this 
ferment has been followed far enough to demonstrate the 
gradual and complete solution of the whole wall-substance 
of the aleurone-layer, since the exposed ends of walls have 
been seen to ‘ melt ’ away after three to four days’ immersion 
in several changes of ferment. 
The sections of the cotyledons of the ungerminated seed 
of Lupinus albus were prepared for the extract of Aspergillus 
as they were prepared for the Barley-extract. Their cell- 
contents were removed with chloroform and extract of 
pancreas ; they were placed in a few drops of ferment- 
solution on glass slides ; the glass slides were enclosed in 
a moist chamber containing chloroform ; and the moist 
chamber was kept in an oven at a temperature of 32 0 to 
34 0 . The phenomena of membrane-solution are almost the 
same here as in the case of the Barley-ferment, except that 
the process requires 'less time. After one to two days the 
membrane appears slightly swollen and laminated. In three 
or four days it has become wholly hyaline except for a very 
narrow zone corresponding to the middle lamella. When 
the middle lamella is split, as occurs very often in this tissue 
in the formation of intercellular spaces, this brilliant, unaltered 
band lies, one half of it, upon each inner lamella. The inner 
lamellae become more and more transparent and gradually 
fade from view. The middle lamella also becomes hyaline, 
and I have seen it disintegrating without residue at the 
