65 
New combe. — Cel lu lose-E nzymes . 
supposed that the cell-walls had lost all their thickenings, 
till I had made the very transparent zone visible by applying 
Bismarck-brown. It seems possible, therefore, that Nadelmann 
misinterpreted appearances, especially since he makes no 
mention of particular caution in observation. 
Whether the wall finally becomes thin or not, is however 
a matter of small moment, and Elfert is certainly wrong in 
supposing that the walls do not contain reserve cellulose. 
Schulze 1 has very recently repeated his former analysis, 
finding that in three weeks’ germination Lupimis angustifolhis 
lost nearly three-fourths of its nitrogen-free material, and 
L. luteus in the same time lost more than one-half. The 
microscopic examination also shows that profound changes 
take place in the substance of the walls. 
D. Extract of Cotyledons of Phoenix dactylifera. 
Date-seedlings were grown in earth till the endosperm had 
been nearly half consumed, when the cotyledons, the petioles 
of the cotyledons, and the remaining endosperm were collected 
into separate groups. The cotyledons were removed from 
the enclosing endosperm by dividing the endosperm longi- 
tudinally by means of a knife and mallet, and then seizing 
the halves of the cotyledon with forceps. About one-third 
of the number of cotyledons were while still fresh rubbed 
in a mortar with infusorial earth and a few cc. of water, 
and the liquid was pressed through a cloth into a paper filter, 
giving a limpid filtrate. The other cotyledons, the petioles, 
and the pieces of endosperm were dried in an oven at 33 0 , and 
each kind of material ground separately in a mill. The 
ground material was in each case extracted with water, the 
liquid filtered off, and to each liquid five times the volume 
of 96 °/ Q alcohol added. After being dried the precipitate 
from the petioles appeared brown, and those from cotyledons 
and endosperm a greyish white. To each dried precipitate 
were added a few cc. of water acidulated to -05 °/ o with 
1 Ueber die Zellwandbestandtheile der Cotyledonen, &c. : Ber. d. d. bot. 
Gesellsch., xiv (1896), 66. 
F 
