New combe .- — Cellulose-E nzymes . 6 7 
of wall between the middle lamella and the layer bounding 
the cell-cavity ; next these limiting layers disappear ; and 
lastly the middle lamella. Control-preparations were made 
with sections in water acidulated with hydrochloric acid to 
the same degree as the ferment-solution. Here, however, in 
the same temperature as before, the walls remained intact for 
a week, when the preparations were discontinued. 
Seeing that the attempts to extract by means of water 
a cytohydrolytic enzyme from the Date had, according to 
published reports, met with but poor success, and Green 1 
having an indication of enzyme-action in a glycerin-extract, 
it was thought best to extract with glycerin the cotyledonary 
material that had already been extracted with water. The 
glycerin used was diluted with water to seven-tenths the full 
strength. Sections of Barley-endosperm freed from starch 
were put into this extract and kept at 33 0 for five days, when 
the walls showed only a thin middle lamella remaining. 
Sections of a cotyledon of a seed of Lupinus albus , killed 
and subsequently treated with pancreas-extract, showed, after 
five days’ immersion in the glycerin-extract of the Date- 
cotyledons, a wholly hyaline condition except in the middle 
lamella. Similar sections from the Barley-endosperm and the 
Lupinus - cotyledon appeared unchanged when immersed for 
five days at the same temperature in glycerin of like strength. 
Several trials with sections from the Barley-endosperm 
showed, as might be expected, that the glycerin-extract of 
the cotyledonary residue was not as active upon the walls 
as was the watery extract previously taken from the same 
material. 
E. Extract of Endosperm of Phoenix dactylifera. 
No one has reported an enzyme in the extract of Date- 
endosperm. Green 2 expressed the belief that a cytohydrolytic 
1 On the Changes in the Proteids in the Seed which accompany Germination : 
Phil. Trans. Roy. Soc. Lond., 178 B, 37. 
2 Phil. Trans., 1. c. 
