7 4 New combe. — Cellu lose-E nzymes. 
enzyme, in thirty-five days or more. In most of the series 
the ferment-solutions were renewed every two or three days ; 
in one series no renewal was made for ten days after the 
beginning of the test. The quantity of ferment-solution 
given to the preparations was, as stated elsewhere, the same 
in every case. 
It having been demonstrated that these ferment-solutions 
which act with almost like intensity on reserve cellulose 
show very unlike powers of dissolving starch-grains, it was 
decided to test the same solutions with starch-paste. A i °/ o 
Wheat-starch-paste was made with distilled water alone, and 
the paste was then acidulated to -ooi °/ o with hydrochloric 
acid. Such a preparation after standing two or three hours 
gives a clear liquid over a flocculent precipitate, and this clear 
liquid is very sensitive to the action of diastatic enzymes. 
When to equal volumes of this starch-paste in test-tubes, 
one-fourth volume of enzyme-solution was added, a little 
chloroform being placed in each tube, and the temperature 
kept at 18 0 , it was found by the iodine-test that with 
Hordeum-e'xXrdLQ.t a brown colour free from purple appeared 
in two hours and ten minutes ; with Aspergillus- ex tract, 
the same shade of brown appeared in five hours and twenty 
minutes ; with A/tezzzT-cotyledonary-extract, in twenty 
hours ; and with PA^zzzT-endosperm-extract, the same brown 
appeared a little later than one hundred and twenty hours. 
Unfortunately the Lupinus- extract was not included in this test. 
However, in another series of preparations, the Lupinus-ex.X.r2iQt 
was directly compared with that of the Phoenix-^ ndosperm. 
The starch-paste was the same as in the preceding series, 
but the proportion of enzyme-solution to the volume of paste 
was as four to ten, and the temperature was kept at 30°. 
Here the brown colour appeared in the Phoenix- endosperm- 
extract between twenty and twenty-one hours after the 
beginning, while in the Lupinus- extract it appeared between 
nineteen and twenty hours from the beginning. Thus it is 
quite certain that had the L upinus-e'xtr&Qt been included 
in the former series of experiments, its period for bringing 
