7 6 Newcombe . — - Cellulose-Enzymes. 
of it by the method given on p. 74 of this paper. With 
the paste prepared by the latter method, the ferment- 
solutions in the proportion of four parts of ferment to ten 
parts of starch-paste were finally brought, by the iodine- 
test, to give the purely brown colour after twenty hours at 30° 
temperature, kept constantly in the presence of chloroform. 
While the ferment-solutions were being brought to a 
common strength, sections from a dry Barley-grain were 
made ready. The sections were cut from the same part 
of the grain, killed, washed in water, those of like dimensions 
and appearance selected by the aid of the microscope, and 
put into solution of extract of pancreas till the starch was 
dissolved. That the pancreas-extract does not visibly affect 
the cell-walls is shown on p. 55 of this paper. With the 
five ferment-solutions and the prepared sections, two series 
of experiments were carried through. A section was put 
into three drops of each ferment on a glass slide, chloroform 
was added, the slides were enclosed in a damp chamber 
containing chloroform, and the chamber was kept in an 
oven whose temperature varied from 30° to 35 0 . The large 
variation of temperature should not have occurred, but it 
could not have affected the general results, since all pre- 
parations were alike subjected to it. Examining these 
preparations at frequent intervals, it was seen that the 
Lupinus-ex tract dissolved the inner lamellae of the starch- 
bearing cells in about nine hours ; the AVWzzz.r-endosperm- 
extract, in nine hours ; the /Vz^/zzhr-cotyledonary- extract, 
in twenty-one hours ; the Aspergillus-extract, in one series 
in ninety-four hours, in the other series in one hundred 
and sixteen hours ; and the Hordeum-extract, also in ninety- 
four and in one hundred and sixteen hours. The middle 
lamella was dissolved by the Lupinus-ex tract in the two 
series in twenty-one hours ; by the P/z^zzzT-endosperm- 
extract, in twenty-one hours 1 ; by the Phoenix-c otyledonary- 
1 The lower limit of ten hours given for the Lupin-extract, and the upper limit 
of thirty-three hours given for the Date-endosperm-extract in my ‘vorlaufige 
Mittheilung’ (Bot. Centrblt. lxxiii), are errors in transcription from my notes. 
