8o New combe. — Celhdose Enzymes. 
has not as yet been found, one may yet be discovered ; and 
on the animal side both saliva and extract of pancreas are 
active toward starch, neutral toward reserve cellulose. The 
discovery of such a plant-diastase would not of course prove 
the point, but it would lend some probability to the view. 
In the second place, relations are such in the cotyledons of 
the Lupin and of the Date that we should expect in the 
extract an admixture of diastase, even if cytase does exist 
there independently ; for in the cotyledons of both plants 
more or less starch is found. In the third place, how is the 
remarkable difference in the behaviour of the extracts 
of the Date-cotyledons and the Date-endosperm to be 
explained ? 
As already shown, though the extract of the cotyledons 
compared with Barley-diastase is extremely feeble in its 
action toward starch, yet it dissolved starch -grains in less 
than one-half the time required by the Date-endosperm- 
extract, and it hydrolyzed starch-paste in one-sixth the time 
required by the endosperm-extract when both ferment- 
solutions acted with like intensity on cell-walls. Yet these 
two extracts are most likely both secreted by the Date- 
cotyledon, and though showing such a difference in properties 
are separated in the plant-tissue at most by a very few cells, 
probably by only a single cell-wall. We have here in the 
Date-seedling the possible conditions presented for securing 
a cytase free from diastase, if such separation ever really 
occurs. We can see no need of diastase in the dissolving 
endosperm, but it may be that a little is carried over by 
diffusion from the secreting cells of the cotyledon. 
In brief the results of this work are the following : — 
1. The enzyme extracted from Aspergillus Oryzae attacks 
reserve cellulose with greater intensity than it attacks starch. 
2. The enzyme from the cotyledons of seedlings of Lupinus 
albus is very strongly cytohydrolytic, but very feebly amylo- 
hydrolytic. 
3. The enzyme from the cotyledons of seedlings of Phoenix 
dactylifera is very strongly cytohydrolytic, and very feebly 
