1 48 Trow. — Observations on the Biology and 
a greater or less extent into fat-globules. The basal wall 
forms in due course and the oogonium produces its eggs in 
the normal manner. The antheridial branches, however, may 
under these circumstances be suppressed altogether, as in 
Fig. 29 ; or if developed they may grow past the oogonium 
without bending towards it ; or they may even grow away 
from it altogether and attain a great length as if they had 
become converted into rhizoids. It is obvious that the 
oogonium must normally exercise a considerable influence 
in determining the nature and direction of growth of the 
antheridial branch. The determination of the nature of this 
influence must remain a subject for further study. 
The eggs in such oogonia develop in every respect in a 
normal manner except that the time required for maturation 
is about doubled. Fertilized eggs mature in five days, un- 
fertilized in ten days even when in oogonia on the same hypha 
in the same moist chamber. 
It was noted in a large number of cases that oogonia such 
as that in Fig. 27, which in all probability were apogamous, 
possessed oospores which germinated in the normal manner 
and produced small sporangia. Two oogonia were kept 
under regular observation for a whole month, from 21/9/98 to 
21/10/98. The eggs, two in number in each case, were 
produced on 21/9/98, they reached maturity in eleven days. 
Fig. 29 represents one of them on 4/10/98. This alone 
developed further — the other one became unhealthy. Fig. 30 
represents a stage in germination. About 17/10/98, the whole 
culture which had been kept in good condition up to this 
time, became unhealthy, and on 21/10/98, when the germinat- 
ing oospores were obviously dead or dying, it was destroyed. 
There can be little doubt that these unfertilized eggs produce 
oospores which are capable under normal conditions of 
developing sporangia and zoospores and giving rise to new 
plants. 
The interest of this observation appears to me to lie in the 
fact that in a group in which apogamy occurs so frequently 
under natural conditions in some of its forms, it may be 
