Ward . — Thames Bacteria , III. 201 
Stab-cultures at 20°. In forty-eight hours the axis has 
developed outgrowths like root-hairs, and a tenuous film with 
a bluish sheen forms above (Fig. 6). In time the film spreads 
as a bluish-white layer above, but the very delicate root-hairs 
grow no further. No liquefaction in a month. The surface 
growth soon forms a continuous opalescent white layer, with 
a peculiar bluish cast on the surface. 
Submerged cultures in gelatine show that it is very slightly 
anaerobic, but grows far better at the surface than in the 
depth. 
Streak at 20°. A thin delicately fimbriated layer in 24-48 
hours, spreading all over the surface in a few days, with the 
margins delicately cut like moss, and the whole film present- 
ing an astonishing resemblance to a frosted window-pane. 
The layer is yellowish-white with iridescent edges and 
surface. No trace of liquefaction in a fortnight. There is, 
however, a slight growth in the gelatine just below the surface. 
The growth itself is matt, like ground glass, and soon acquires 
metallic iridescence on the surface like glass which has been 
buried (Fig. 5). 
Agar at 35 0 . A yellowish-white streak with slightly 
spreading thin margins, cut and fimbriated, rapidly develops 
in 24-48 hours and then grows no further. It then remains 
unaltered for a month. 
At 25 0 the growth is much better, as a delicate white film 
spreading all over in 24-48 hours, with radiating filaments 
from the streak forming moss-like outgrowths as on gelatine 
(Fig. 7). This also remains stationary after about 4-5 days, 
and appears unaltered in five weeks. 
Potato. At 35° hardly any growth perceptible. At 25 0 
a faint whitish film in 24-48 hours, but it does not spread. 
The potato acquires a bluish-grey hue in a week, and the thin 
dryish film then shows up a little clearer, but even in the best 
cases it is an indistinct grey-white film. It consists entirely 
of cocci, isolated for the most part. 
Broth. At 35 0 rapidly turbid all through, and remains so 
for many days. No ring or veil, but a white deposit accumu- 
