204 Ward . — Thames Bacteria , III ’ 
thick. Decolorized by Gram’s method. Plate-cultures at 
1 2-1 5 0 give typical colourless, contoured, typhoid-like colonies, 
growing very slowly. 
Plates at 20° showed white flecks | mm. in diameter in two 
days. These have a spider-like appearance under the lens, 
and are composed of moss-like tufts of filaments and tresses 
from zoogloea-centres (Fig. 1 1 a). On the third day the 
corkscrew-like zoogloea-tresses radiate to the surface and 
form queer spider-like groups, and thin contoured fronds, up 
to 2 mm. in diameter (Fig. n b). Many colonies present 
dendritic or Moss-agate figures, but as the filaments reach the 
surface they flow out into flat, flame-like and delicately 
contoured lobes. No signs of softening on the fifth day 
(Fig. ii). 
Stab-cultures at 12-15 0 give white dots in the tunnel, and 
an extremely thin hyaline frond-like film above in two days. 
In five days the film is still extremely tenuous, and the growth 
very slow. 
In two days at 22° a beautiful and very delicate transparent 
frond, and irregular fine root-hair-like radiations from the 
tunnel. It grows in slightly acid gelatine also, but best in 
alkaline. On the sixth day the film is still exquisitely thin 
and transparent. 
After six weeks the centre has sunk a little, but no liquid 
results : it is a dry hole 3-4 mm. deep and same diameter. 
Streak at 20°. An extremely delicate thin and transparent 
film in two days, with whiter dots along the streak. In 
a week the film has spread nearly all over, but is scarcely 
visible at the transparent jagged edges, where it is flush with 
the gelatine and broad. 
Agar at 34 0 . White spots along the streak in twenty-four 
hours. In forty-eight hours these have extended rapidly and 
are coalescing, like very thin scales, to a broad, rather waxy 
patchy streak. In four days it forms a very thin, flat, 
shining, yellowish film, with blue iridescent margins, and 
radiate zones. The streak thickens later to a shining, dirty- 
white, gum-like layer. 
