216 
Ward . — Thames Bacteria , ///. 
colonies as follows. Gram’s method decolorizes it, but it is 
easily stained by ordinary methods. 
Plates at 12-15° C. Yellowish-grey, granular, circular discs, 
often with irregular edges, appeared in two to three days. On 
the fourth day liquefaction began, each colony being merely 
a zoned brownish circular disc about 1-2 mm. diameter, the 
edges of each colony being fimbriated or even with irregular 
tresses. Liquefaction very rapid on the fourth day, the small 
colonies — white to the unaided eye — retaining their circular 
shape as they float. Under the -g- each was a grey disc, 
hollowing out in the centre, and with fimbriated edges 
(Fig. 24). 
After-plates gave the same dimensions, i. e. ordinary round- 
ended rodlets 1*5-4// x 1 //. The thickness not uniform, but 
in some cases 0*75-1 //. 
After three months in culture, plates at 15-18° showed 
white-grey spots in three days, each sunk in a shallow lenticular 
depression. Under the J the submerged colonies are moruloid, 
tawny, granular : as they emerge they thin out with anthrax- 
like tresses and fimbriated edges. On the fourth day they 
are about 1-4 mm. diameter across the lenticular depression, 
the ochre-coloured central eye being solid, or 1 -zoned, and 
under the f the zone is found to be due to clouds around the 
more solid centre. Others are masses of tresses, with fimbriated 
edges. They lift as a whole from the depression. 
On the fifth day the lenticular depressions are coalescing : 
they measure 5-8 mm. in diameter. The central ochre-yellow 
eye resembles a sieve, and is composed of dense and less dense 
clouds. The fluid in the depression is turbid. In a week the 
plate is nearly liquid. 
Stab-cultures at I2-I5°C. Rapidly forms a thistle-head 
funnel, and liquefaction occurs and spreads to the sides. 
Repeated tough whitish veils form on the surface, and fall 
successively, and the resulting deposit is very shiny and tough. 
The top of the liquid may be clearer, or it may be more 
turbid above (Fig. 25). In a fortnight nearly all the gelatine 
is liquefied with a white ring and veil above: dense clouds fall 
