234 
Ward . — Thames Bacteria , III. 
the flat floor, under a nearly clear yellow liquid. The lique- 
faction does not quite reach the bottom of the still slender 
tunnel, but it gradually extends to the bottom of the tube in 
the course of three or four months. 
At 1 8-20° the thin film above is rapidly developed in forty- 
eight hours, with a yellow liquefying centre, and the axis 
is surrounded with a pale cloudiness as in No, 23 under the 
same conditions. On the third day yellow cloudy liquefaction 
sets in above, and cloudiness throughout the solid gelatine, 
exactly as in No. 23 ; and so also on the fifth day, i. e. the 
axis fades, and a watery layer with yellowish cloudy masses 
occurs all over the top. 
At 24 0 rapid liquefaction as a cloudy yellowish mass above, 
and clouds in the gelatine. 
Streak at T5 0 C. Rather rapid ochre-yellow streak, scoop- 
ing in forty-eight hours and with a delicate fringed filmy 
border to the scooped channel. The orange mass slides down 
into the viscid liquid at base. In fourteen days nearly all is 
liquid, bright orange deposit and a turbid liquid exactly like 
No. 1 7, but with no veil. All liquid in twenty-two days : 
deep orange deposit. 
At 23° the behaviour is almost exactly the same-rapid 
scooping of a channel off which the mass runs, leaving a thin 
fringe of radially arranged film attached to the gelatine. On 
the third day the action is somewhat slower than at I 5 °,but in 
longer cultures (fourteen days) it is a little more rapid : other- 
wise exactly the same. All liquid in twenty-two days, and 
deep orange deposit, consisting of cocci only (examined in 
water). 
Agar at 34 0 C, showed no growth. 
At 25 0 the growth is also slow and thin. In seven days 
a scarcely visible film, smooth, glistening and transparent, and 
only visible owing to its faint iridescence, and yellow colour 
when viewed edge on : a yellowish deposit in the drainage. 
Even in fourteen days the layer does not thicken, but on pass- 
ing the loop over one collects a yellow mass. 
At 22° a similar thin orange film grew, and orange deposit. 
