Ward . — Thames Bacteria , III. 
235 
In about a week the glassy layer can be made out to be 
orange-yellow, and the deposit is deep orange. Older cultures, 
kept a month or more at 12-15°, show a more distinct growth 
— still thin, but more opaque and evident, and scraping off as 
a thin orange paste. 
After being three or four months in culture, this form 
became so weak that it hardly grew at all on agar at 15°, 
25°, or 30° if infected from agar; if, on the other hand, 
infected from old gelatine-cultures it grew very well at 15° as 
a glassy film to type. 
The failures are not due to aeration ; no growth was got in 
anaerobic conditions in fourteen days at 22-25° C. 
After cultures through broth and again isolated on gelatine, 
however, the agar-growths at 18-21° were as good as ever: 
yellow gummy layers in forty-eight hours with pale lilac 
iridescence and spreading all over. This lilac iridescence is 
sometimes very marked about the fourth day. In eight days 
the lilac tinge has gone, the thickish layer is ochre-yellow, 
gum-like with jagged thin edges, and deposit yellow. Much 
more yellow than No. 23. 
On Potato at 22° C. it often refuses to grow, but in some 
cases a slight golden-yellow patch forms, and in a few cases 
this became a dull orange fairly thick patch in seven days. 
It does not spread, and becomes dull, dry and wax-like. At 
25° the best growths were very slight, pale golden-yellow, 
hardly extending beyond the streak. No growth could be 
got on potato at 34° C. 
Broth at 25° C. Slightly turbid in forty-eight hours. In 
a week a slight turbidity and faint ochre deposit. In a month 
the same shimmering turbidity and orange deposit. No growth 
at 35°, but at 18° it is as good as at 25°. The colour of the 
deposit deepens to red-orange, and the broth becomes sherry- 
colour. 
Milk at 25° showed no signs in fourteen days, but traces of 
separation were observed on the fifteenth, and in three weeks 
a stiff clot had formed ; the reaction faintly acid. In a month 
all the casein was down as a coagulum below a straw-coloured 
