236 Ward . — Thames Bacteria , III. 
liquid ; but no signs of peptonization could be detected in 
six weeks. 
Ghicose at 25 0 . No results beyond a very faint shimmer 
about the ninth day, and an extremely slight, hardly white 
deposit. 
No. 7 was found by Dr. Kanthack to be non-pathogenic to 
guinea-pigs. 
No. 7 was revived from an agar-tube thirteen months old 
(May 28 to June 14 of following year), but it was so weak 1 
that I could not get the plate-colonies beyond the stage of 
golden-yellow circular colonies of the A type — minute points 
not more than 0*5 mm. diameter just sinking into the non- 
liquefied gelatine in fourteen days, at 20°, and then growing 
no larger ; liquefaction never took place. 
It certainly appears that here we have a weakened liquefy- 
ing form becoming non-liquefying. If we compare No. 7 with 
No. 23 the following points of difference come out. 
No. 7 breaks up to shorter rodlets. In the plate-colonies 
there are slight differences of colour and rate of liquefaction 
and pigmentation only. 
In stab-cultures also the differences are chiefly as to depth 
of colour, and No. 23 forms a veil. 
Agar-cultures of No. 7 differ little in colour and thickness, 
and it appeared somewhat weaker, and this feebleness 
appeared on potato also. 
But revival in broth was easy at first. In broth No. 23 
differs by its veil and greyer deposit. 
In milk No. 7 slowly coagulated but did not peptonize, and 
in this is distinct from No. 23. 
These differences can hardly be insisted on as distinctive, 
and I therefore regard No. 7 as the same as No. 23, and both 
as identical with Zimmermann’s B. radiatus. 
I have already referred to the connexion between these 
forms and the Proteus of Hauser, and this is strengthened by 
the evident preference for peptones and broth as against 
1 i. e. I infer it is weakened, by its behaviour in gelatine, and because no 
attempts to invigorate it by broth-cultures succeeded. 
