238 Ward . — Thames Bacteria , III. 
sinking spot above, and dots in tunnel. No cloudiness. On 
the third day a shallow funnel of liquefaction above : no 
change in tunnel. Thistle-head in five days, white flocculent 
mass ; axis with yellowish-white dots, sword-shaped. 
At 24° also much slower than Nos. 7 and 23. 
Streak at 15 0 — very slow, merely shows bluish- white in 
forty-eight hours. 
At 23 0 also slow : mere streak in forty-eight hours. 
Agar at 34 0 . No growth. At 25°a thin, smooth, yellowish- 
white layer, pinkish in some lights, covers the agar in five 
days, and an abundant yellowish-white deposit falls. It is 
like No. 23. 
At 22° rapidly forms a thin, glassy, yellowish-white film 
with a greenish cast. In three days the fimbriated edges 
are remarkably iridescent : yellowish precipitate in the slightly 
turbid drainage. In four days, a greenish-white glistening 
layer — the green sheen only in some lights. 
The layer, when scraped off with a loop, is dirty-white. 
Exactly the same difficulties as with No. 7 occur as regards 
old agar-growths losing power. No growth under anaerobic 
conditions in fourteen days at 22-25 0 C. 
At 18-21 0 a greenish-white flecked layer with pale lilac 
iridescence in some lights, rapidly spreads in forty-eight hours. 
The curious faintly marbled or flecked appearance was some- 
times marked about the fourth day ; it has a slight iridescence 
of greenish and lilac. In eight days, much like No. 23, but 
edges less jagged, and green to lilac iridescence still marked. 
Yellow-white deposit. 
Potato at 3o°C. No growth. At 25 0 a greyish film in 
twenty-four hours becoming drier and not spreading. The 
growths on potato at 22° varied considerably according as 
the medium was alkaline or not. On ordinary potato a pale 
ochre patch formed in forty-eight hours, and this spread 
but little, as a dry small ochre streak with a whitish rim : 
on alkaline potato a thin gummy ochre film formed in forty- 
eight hours, and in three days this was flowing all over, 
with lilac margins. This pink-lilac hue spreads as the 
