240 
Ward . — Thames Bacteria , III. 
granular firm dots, pale and very transparent under the 
These are apt to stick to the glass (Fig. 49). 
On re-sowing and obtaining less crowded plates, the colonies 
form yellow-eyed, sinking circular colonies, with spider-like 
or dumb-bell-shaped zoogloea-mass in centre, and floating 
islets around by the end of a week. Most of the surrounding 
clouds or islets are spider-like zoogloeas (Fig. 50). 
In three weeks the gelatine is softened, and the colonies 
pale yellow. Sour smell. Plates of No. 17 after being some 
months in culture give, at 18-31°, colonies just visible in forty 
hours, under these are moruloid, or spiky, or spider-like 
zoogloea-forms, yellowish. 
On the third day (see Figs. 50 and 51), while most of the 
colonies are as above, but larger, others emerge as plates 
1 mm. diameter with spider-like zoogloeas in the centre. On 
the fourth and fifth days the small yellow dots present similar 
features, with much variation in details, and the yellow colour 
is more pronounced ; some are still moruloid or gland-like, 
and submerged ; others are cloudy centres with outlying 
nebulae, and consist of much branched, dendritic, zoogloea- 
tresses ; others are contoured plates as before, with zoogloea 
in the centre. In the course of two or three days more lique- 
faction sets in, and the floating cloud-like colonies are yellow 
with a deep chrome-yellow central eye, reminding one of 
a weak form of No. 35. 
The film is more frondose at the edges, however, and sink- 
ing begins about the fourteenth day, and a yellow frondose 
floating mass remained above, like egg-yolk. 
Stab-cultures at 13-15°. Begins by forming an extremely 
thin yellow film above, and pale yellow dots in the tunnel in 
three days. In six days the yellow centre is surrounded by 
a bluish or pale purple iridescent border : slimy and viscous 
to the needle. 
At 18-31° a slight sinking yellowish spot is formed above 
in forty-eight hours, and short root-hairs along the axis 
(Fig. 53 a). Small funnel in three days. In five days rings 
around the axis may replace the root-hairs (Fig. $lb). 
