292 
Lam q — T he Prothallus 
. <3 
shape by the pressure of the adjoining hyphae. It was usually 
found close to the vacuole, but sometimes against the cell- 
wall. No indication of septa in the hyphae has been seen, 
though a large number of sections have been examined with 
this object in view. Their infrequency may therefore be 
considered to be probable ; though owing to the difficulty 
of examining a long filament of the Fungus, their absence 
cannot be definitely asserted. Small oval nuclei occur at 
intervals in the filament. The intercellular hyphae resembled 
those found within the cell-cavities. 
The appearance of the endophyte was found to present 
differences according to the region of the prothallus in which 
it occurred, and differences of a similar nature were noted 
when fertilized prothalli were compared with those in which 
marginal growth continued. The important factor common 
to both these cases appears to be the age of the tissue in 
which the Fungus is found. 
Taking first the case of an unfertilized prothallus, the 
appearance of the Fungus may be described as it is seen in 
/ a section on passing from the margin towards the more central 
regions of the prothallus. For a short distance from the 
small-celled meristem at the margin, the tissues are free from 
Fungus. In the corresponding cells a little further from the 
margin, fungal filaments are found in the protoplasmic body, 
running for the most part parallel to one another. ’ None but 
these vegetative hyphae are present in this region, and both 
the cells and the mycelium appear to be quite healthy. 
Passing to the cells slightly further from the margin, in 
addition to the ordinary hyphae, bodies of peculiar form are 
found in them ; the nature of these will have to be discussed 
later, but they may be termed multinucleate vesicles. A 
number of these are to be seen in the portion of a vertical 
section represented in Fig. 6. They were found to be most 
numerous in the band of tangentially extended cells, but 
they occur occasionally in the cells of the palisade-layer ; 
they have not been observed between the cells. Early 
stages of these vesicles are represented in Fig. 31 a and b, and 
