Biffen . — A Fat- Destroying Fungus . 367 
the margin of a vacuole ; while sometimes, where a nucleus 
appeared to have just divided, the daughter-nuclei were 
separated by a large vacuole (Fig. 3). 
In hanging-drop and plate cultures, chains of chi am y do- 
spores were developed from the mycelium which produced 
microconidia, especially as the gelatine began to dry. The 
chlamydospores are spherical, or slightly elliptical, in shape, 
with thick brown walls, and contain many of the proteid 
reserve bodies already mentioned (Fig. 4). O11 germination 
they gave rise to a mycelium which again produced micro- 
conidia. 
These were the only forms of spores produced in the gela- 
tine-cultures, but macroconidia were formed in the cultures 
on coco-nut endosperm and Brazil-nuts. Like the micro- 
conidia, they are abstricted terminally and singly from the 
hyphae. At first the young macroconidia cannot be dis- 
tinguished from microconidia, but they soon become sickle- 
shaped ; and when they have reached their full size they 
divide by transverse walls, so that ultimately each is composed 
of three or four cells each with its own nucleus (Fig. 5). Like 
the microconidia, they contain considerable quantities of oil. 
Germination may occur from any one, or from all three or 
four cells. The mycelium formed is similar to that formed 
by the microconidia and the chlamydospores ; but it is charac- 
terized by having many of its hyphae united into a network 
by the fusion of short lateral processes, very like those put 
out by conjugating Spirogyra (Fig. 6). 
In cultures four weeks old the mycelium in the endosperm 
had given rise to a stroma formed from strands of hyphae 
running parallel with one another, and united on the surface 
of the blocks by branches running at right angles (Fig. 7). 
These branches interlace to such an extent that the mode of 
development is soon lost sight of, and the stroma appears to 
be composed of hyphae running in every direction. When 
mature it is chocolate-brown in colour and fleshy in texture. 
Its thickness is from one to two mms. 
Even in this early stage rudimentary perithecia can be 
