3 66 
Kauffman. — A Contribution to the 
Solutions were made by mixing a *05 per cent, haemoglobin solution 
and a -2 per cent, solution of the inorganic salt desired, using half of each 
solution ; this gave a solution containing in each case *025 per cent, haemo- 
globin and -i per cent, of the salt. The following salts were tried : K 3 P 0 4 , 
xKH 2 P 0 4 , xNa 2 HP 0 4 , Ca(NO s ) 2 , xNH 4 NO s , KN 0 3 , x(NH 4 )P 0 4 (Ca) 3 
(P 0 4 ) 2 , xNaH 2 P 0 4 ', K 2 S 0 4 , xZnCl 2 . Also peptone in -02 per cent, solution. 
Those marked with an x showed few if any oogonia. The results are based 
on the first eight days’ growth : — 
(a) Solutions with -05 per cent, haemoglobin and no salts were tried first. 
Here the oogonia were abundant, with many oospores in each oogonium ; 
the hypogynous cells were present but with hardly any side-branch. 
(b) Haemoglobin and peptone. Oogonia very abundant with 2-10 
oospores in each oogonium ; the hypogynous cell beneath each oogonium 
had no side-branches, but there were projections into the oogonium. 
(c) Haemoglobin and potassium phosphate. Oogonia abundant, but 
the oospores did not always mature ; in such cases there was always 
a great abundance of antheridial filaments, which arose either from the 
hypogynous cell or from the neighbouring mycelium ; hypogynous cell was 
either absent or present. 
(d) Haemoglobin and calcium nitrate. Oogonia medium abundant ; 
hypogynous cell was present ; side-branches short or wanting, not developing 
into antheridia. 
(e) Haemoglobin and potassium nitrate. Oogonia abundant with 1-10 
oospores ; hypogynous cell either present or absent ; antheridial branches 
present, often luxuriant around the disintegrating oogonia. 
(/) Haemoglobin and calcium phosphate. Oogonia abundant, with 
one to many well-formed oospores ; hypogynous cell always present, 
sometimes several ; no antheridial branches. 
Another set of cultures contained -05 per cent, haemoglobin, and 
the same series of salts as before, including ammonium tartrate and rubidium 
hydroxide. The results noted were obtained during the first eleven days of 
development : — 
(a) Solutions with *i per cent, haemoglobin were used, containing 
no salts. Oogonia very abundant with 3-1 5 oospores in each oogonium ; 
always with a well-formed hypogynal cell which is only rarely branched, 
and then the side-branch is short. A few cases of additional hypogynous 
cells were seen. 
(b) Haemoglobin and -i per cent, peptone. Oogonia abundant, with 
3-10 well-formed oospores; hypogynous cell always present, sometimes 
two cells ; no antheridial branches. 
( c ) Haemoglobin and -05 per cent, potassium phosphate. Oogonia 
abundant, with three somewhat disintegrated oospheres, but with antheridial 
filaments in great profusion around the oogonia. 
