37° 
Kauffman. — A Contribution to the 
former being usually transformed gemmae. Both leucin and haemoglobin 
series of cultures were tried. The sporanges were of the Saprolegnia type 
and emptied in the same manner. 
The plant was obtained from mosses collected in a ditch near a peat 
bog at Chelsea, Mich., November n. Since the mycelium did not respond 
readily when transferred to pure water in the formation of zoospores, the 
pure culture was made by transferring a single gemma as Klebs had done. 
This germinated by sending out from its anterior end numerous branches, 
while the hyphae at the end where it had been attached shrivelled up. 
Gemmae were formed in greater or less numbers in nearly all the 
solutions of the series of haemoglobin. The solutions tried contained 
•025 per cent, haemoglobin with *i per cent peptone, laevulose, glucose, 
K 3 P0 4 , KN0 3 , CaHPOJ, Ca(N0 3 ) 2 , K 2 S0 4 , NaCl, Cs(OH) 2 respectively. 
Various combinations of these were also tried, but without success. With 
Cs(OH) 2 no gemmae were formed, while the vegetative growth was good. 
Leucin was used with Ca 3 (P0 4 ) 2 , Ca(N0 3 ) 2 , KN0 3 ', K 3 P0 4 , and 
MgS0 4 . In all these leucin solutions, only gemmae were formed, often 
in great abundance. In shape the oogonia were often globular, as if they 
were oogonial initials, especially in the haemoglobin-laevulose and potassium 
phosphate and potassium sulphate solutions. The clusters of gemmae are 
short and much branched in many cases. 
I. Saprolegnia , sp. This species reacted similarly to L, except that in 
one culture oogonia were seen. This occurred on a pure fly culture, but 
they remained immature and disintegrated, and no determination was made 
as to the species. The oogonia which were noticed were densely covered 
by antheridia, even in the rather young condition, and this fact makes it very 
unlikely that we had another specimen of S. mixta . The antheridia were 
both diclinous and androgynous. 
The material was obtained from a jar containing Elodea, and brought 
from the Huron river in September. A culture from a single zoospore was 
obtained January 10. The series of cultures were similar to those used with 
the preceding species. Haemoglobin with the various salts always produced 
abundant gemmae, which were usually large, fusiform or spherical in shape. 
With rubidium hydroxide no gemmae appeared ; only a luxuriant vegetative 
growth seemed possible. With leucin and the series of salts the results were 
practically the same as in the haemoglobin cultures. 
K. In contrast to the two preceding forms, this species produced 
sporangia in abundance. These sporanges were often clavate to narrowly 
ovate, and were arranged irregularly in acropetal series. This indicates 
a Pithyopsis . No oogonia were seen in any of the cultures, although 
entire series of both haemoglobin and leucin cultures were carried through. 
As in the case of the two preceding forms, gemmae were present in 
all of the cultures except in that of rubidium hydroxide ; in the latter 
