432 S toward. — On Endospermic Respiration in Certain Seeds - 
Exp. 30. Intact seeds steeped in (1) 0-5 % mercuric, chloride, 1 hour; embryos then 
excised, and spermoderm and aleurone layer filed off. Pure endosperms 
then steeped in 
(2) water, 
20 hours. 
< 
No. of 
objects. 
Wt. 
grms. 
Vol. 
c.c. 
Temp. 
C.° 
After hours 
of . 
respiration. 
6T> 2 %. 
0 ,%. 
Mgms. 
co 2 . 
15 
2-6738 
4-°5 
1 8° 
8 
0-00 
19-56 
0-00 
3-75 
20° 
24 
0-00 
18-24 
0-00 
Pure Endosperms derived from Germinated Seeds. 
Exp. 31. Intact seeds steeped in (1) o-i % HgCl 2 , \ hour; (2) water, 33 hours; and 
then (3) germinated in sterilized sand, 64 hours; radicles and plumules 
amputated, and seeds placed in o«i % HgCl 2 , 10 minutes. Then, after wash- 
ing with sterilized water and drying, spermoderm and aleurone layer filed off. 
15 1-9194 2-85 21 0 8-J o-86 18-78 0-002661 0-70 
2-35 21 0 26 2-394 15.34 0-001997 0-51 
7. Experiments with Seeds of Ricinus. 
Only three experiments were made with the endosperms of this seed, 
the variety employed being R. Zanzibarensis. The interest associated 
with these experiments with this seed, lies in the fact that its endosperm 
is universally regarded as possessing vitality. 
8. Table. Experimental Data, Ricinus. 
Exp. 
1. Intact seeds steeped in water, 19 \ hours; 
embryo then excised, and 
endosperm sliced into four pieces. 
No. of 
objects. 
Wt. Vol. Temp. A f terhours 
orms cc C° . uu 2 /o' 
& ’ ‘ ’ respiration. 
4 ,%- 
M C S Z co */°>- 
1 
1-297 4-4 25 0 4 ll-o8 
1-406 
0-1690 0-59 
Exp. 
2. Dry unsteeped seeds degermed, and endosperm sliced into four pieces. 
1 
0-609 — 15 0 5 0-00 
20-46 
0-00 
16 0 25 o-oo 
18-51 
0-00 • — 
Exp. 3. Outer integument removed; seed steeped in saturated aqueous chloroform, 
24 hours; embryo then removed, and endosperm sliced into four pieces. 
1 0-985 2-9 14 0 5 3-619 13-15 0-03983 0-52 
Experiment re-established : 
3*7 
15 
234 
12-28 
1.930 
0-03656 0-67 
